Acquired contractile ability in human endometrial stromal cells by passive loading of cyclic tensile stretch.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Tensile Strength*; Endometrium/*cytology ; Myocytes, Smooth Muscle/*cytology ; Stromal Cells/*physiology; Actins/antagonists & inhibitors ; Actins/metabolism ; Adult ; Cells, Cultured ; Collagen Type I/metabolism ; Cyclic AMP/metabolism ; Female ; Gene Expression Regulation ; Humans ; Ionomycin/pharmacology ; Isoquinolines/pharmacology ; Middle Aged ; Muscle, Smooth ; Oxytocin/pharmacology ; Peristalsis ; Stromal Cells/cytology ; Stromal Cells/drug effects ; Sulfonamides/pharmacology ; Up-Regulation

The uterus plays an important and unique role during pregnancy and is a dynamic organ subjected to mechanical stimuli. It has been reported that infertility occurs when the peristalsis is prevented, although its mechanisms remain unknown. In this study, we found that mechanical strain mimicking the peristaltic motion of the uterine smooth muscle layer enabled the endometrial stromal cells to acquire contractility. In order to mimic the peristalsis induced by uterine smooth muscle cells, cyclic tensile stretch was applied to human endometrial stromal cells. The results showed that the strained cells exerted greater contractility in three-dimensional collagen gels in the presence of oxytocin, due to up-regulated alpha-smooth muscle actin expression via the cAMP signaling pathway. These in vitro findings underscore the plasticity of the endometrial stromal cell phenotype and suggest the possibility of acquired contractility by these cells in vivo and its potential contribution to uterine contractile activity. This phenomenon may be a typical example of how a tissue passively acquires new contractile functions under mechanical stimulation from a neighboring tissue, enabling it to support the adjacent tissue's functions.

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0 (ACTA2 protein, human)
0 (Actins)
0 (Collagen Type I)
0 (Isoquinolines)
0 (Sulfonamides)
50-56-6 (Oxytocin)
56092-81-0 (Ionomycin)
E0399OZS9N (Cyclic AMP)
M876330O56 (N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide)

Date Created: 20200604 Date Completed: 20201217 Latest Revision: 20210602

20221213

PMC7265371

10.1038/s41598-020-65884-3

32488068