Testing the Effectiveness of Curcuma longa Leaf Extract on a Skin Equivalent Using a Pumpless Skin-on-a-Chip Model.

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  • Additional Information
    • Source:
      Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
    • Publication Information:
      Original Publication: Basel, Switzerland : MDPI, [2000-
    • Subject Terms:
      Lab-On-A-Chip Devices*; Curcuma/*chemistry ; Fibroblasts/*drug effects ; Keratinocytes/*drug effects ; Plant Extracts/*pharmacology ; Skin/*drug effects; Cells, Cultured ; Coculture Techniques ; Cosmetics/pharmacology ; Dermis/drug effects ; Epidermal Cells ; Epidermis/drug effects ; Fibroblasts/cytology ; Fibronectins/metabolism ; Humans ; Immunohistochemistry/methods ; Keratinocytes/cytology ; Microfluidics/methods ; Plant Leaves/chemistry
    • Abstract:
      The in vitro tests in current research employ simple culture methods that fail to mimic the real human tissue. In this study, we report drug testing with a 'pumpless skin-on-a-chip' that mimics the structural and functional responses of human skin. This model is a skin equivalent constituting two layers of the skin, dermis and epidermis, developed using human primary fibroblasts and keratinocytes. Using the gravity flow device system, the medium was rotated at an angle of 15 degrees on both sides so as to circulate through the pumpless skin-on-a-chip microfluidic channel. This pumpless skin-on-a-chip is composed of upper and lower chips, and is manufactured using porous membranes so that medium can be diffused and supplied to the skin equivalent. Drug testing was performed using Curcuma longa leaf extract (CLLE), a natural product cosmetic ingredient, to evaluate the usefulness of the chip and the efficacy of the cosmetic ingredient. It was found that the skin barrier function of the skin epidermis layer is enhanced to exhibit antiaging effects. This result indicates that the pumpless skin-on-a-chip model can be potentially used not only in the cosmetics and pharmaceutical industries but also in clinical applications as an alternative to animal studies.
    • References:
      J Dermatol Sci. 2006 Sep;43(3):159-69. (PMID: 16887338)
      Arch Dermatol Res. 1979 Aug;266(1):33-41. (PMID: 507927)
      Int J Pharm. 2000 Aug 10;203(1-2):211-25. (PMID: 10967443)
      J Invest Dermatol. 1984 May;82(5):453-7. (PMID: 6392430)
      J Natl Cancer Inst. 1991 Jun 5;83(11):757-66. (PMID: 2041050)
      Exp Cell Res. 1997 Jun 15;233(2):330-9. (PMID: 9194495)
      Arch Toxicol. 2008 Apr;82(4):211-36. (PMID: 18322675)
      J Cell Sci. 2009 May 1;122(Pt 9):1285-94. (PMID: 19386895)
      Dermatology. 2000;201(1):15-20. (PMID: 10971053)
      Cancer Res. 1988 Feb 1;48(3):589-601. (PMID: 3335022)
      Oncotarget. 2014 Sep 30;5(18):8402-15. (PMID: 25268742)
      Biology (Basel). 2014 May 30;3(2):345-67. (PMID: 24887773)
      J Invest Dermatol. 2003 Aug;121(2):231-41. (PMID: 12880413)
      J Anat. 1998 Jul;193 ( Pt 1):1-21. (PMID: 9758133)
      J Invest Dermatol. 2004 Jul;123(1):13-22. (PMID: 15191537)
      J Invest Dermatol. 1984 May;82(5):449-52. (PMID: 6210326)
      Eur J Pharm Biopharm. 2005 Jul;60(2):167-78. (PMID: 15913972)
      J Invest Dermatol. 1992 Mar;98(3):343-50. (PMID: 1372028)
      Trends Mol Med. 2008 Jan;14(1):20-7. (PMID: 18068483)
      Biomaterials. 2005 Mar;26(9):979-86. (PMID: 15369686)
      J Cell Sci. 2004 Mar 15;117(Pt 8):1351-63. (PMID: 14996912)
      Free Radic Biol Med. 1995 Aug;19(2):227-50. (PMID: 7649494)
      Mol Cancer Ther. 2004 Jul;3(7):763-72. (PMID: 15252137)
      Int J Pharm. 2017 Mar 15;519(1-2):178-185. (PMID: 28104405)
      J Invest Dermatol. 2000 Oct;115(4):708-13. (PMID: 10998148)
      J Invest Dermatol. 1990 Jun;94(6 Suppl):128S-134S. (PMID: 2161886)
      Biomaterials. 2005 Aug;26(23):4847-55. (PMID: 15763264)
      Carcinogenesis. 2007 Aug;28(8):1765-73. (PMID: 17522064)
      J Pathol. 2004 Nov;204(4):355-66. (PMID: 15495218)
      Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):6906-10. (PMID: 1379726)
      J Biol Chem. 2002 May 24;277(21):19122-30. (PMID: 11889133)
      Bioessays. 2002 Sep;24(9):789-800. (PMID: 12210515)
      J Invest Dermatol. 1980 Sep;75(3):270-4. (PMID: 6997401)
    • Grant Information:
      HRF-201911-015 Hallym University
    • Contributed Indexing:
      Keywords: Curcuma longa; antiaging; human-skin equivalents; microphysiological system; pumpless skin-on-a-chip
    • Accession Number:
      0 (Cosmetics)
      0 (Fibronectins)
      0 (Plant Extracts)
    • Publication Date:
      Date Created: 20200604 Date Completed: 20210330 Latest Revision: 20210330
    • Publication Date:
      20221213
    • Accession Number:
      PMC7312991
    • Accession Number:
      10.3390/ijms21113898
    • Accession Number:
      32486109