IL-21 + CD4 + T helper cells co-expressing IFN-γ and TNF-α accumulate in the joints of antinuclear antibody positive patients with juvenile idiopathic arthritis.

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  • Additional Information
    • Source:
      Publisher: Academic Press Country of Publication: United States NLM ID: 100883537 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1521-7035 (Electronic) Linking ISSN: 15216616 NLM ISO Abbreviation: Clin Immunol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Orlando, FL : Academic Press, c1999-
    • Subject Terms:
      Antibodies, Antinuclear/*immunology ; Arthritis, Juvenile/*immunology ; Interferon-gamma/*metabolism ; Synovial Fluid/*immunology ; T-Lymphocytes, Helper-Inducer/*immunology ; Tumor Necrosis Factor-alpha/*metabolism; Arthritis, Juvenile/pathology ; Child ; Child, Preschool ; Female ; Humans ; Interleukin-17/metabolism ; Interleukins/metabolism ; Male
    • Abstract:
      Juvenile Idiopathic Arthritis (JIA) is currently classified into seven subgroups. Recently, antinuclear antibody (ANA) positive JIA patients were suggested to encompass a clinically homogenous new subgroup. CD4 + T helper (Th) cells play an essential role in JIA pathogenesis. Herein, we analyzed cytokine expression in synovial fluid (SF) CD4 + Th cells of JIA patients by using flow cytometry and compared cytokine patterns between JIA subgroups. We could show increased frequencies of IL-21 expressing CD4+ Th cells in the joints of ANA+ Oligo-/Poly-JIA patients, which co-expressed the Th-1 cytokines IFN-γ/TNF-α. In contrast, frequencies of IL-17 expressing cells were lowest in the joints of ANA+ Oligo-/Poly-JIA but enriched in that of ERA-JIA patients. This is the first description of a diverse SF Th cell cytokine pattern in different JIA subgroups. Additionally, we could define IL-21 as an effector cytokine expressed in SF Th cell in a significant proportion of ANA+ JIA patients.
      Competing Interests: Declaration of Competing Interest The authors declare that they have no conflict of interests.
      (Copyright © 2020 Elsevier Inc. All rights reserved.)
    • Contributed Indexing:
      Keywords: Antinuclear antibodies (ANA); Cytokine pattern interleukin 21 (IL-21); Juvenile idiopathic arthritis (JIA); interleukin 17 (IL-17)
    • Accession Number:
      0 (Antibodies, Antinuclear)
      0 (IFNG protein, human)
      0 (IL17A protein, human)
      0 (Interleukin-17)
      0 (Interleukins)
      0 (TNF protein, human)
      0 (Tumor Necrosis Factor-alpha)
      82115-62-6 (Interferon-gamma)
      MKM3CA6LT1 (interleukin-21)
    • Publication Date:
      Date Created: 20200603 Date Completed: 20210201 Latest Revision: 20210201
    • Publication Date:
      20240829
    • Accession Number:
      10.1016/j.clim.2020.108484
    • Accession Number:
      32485239