Chemical renal denervation-induced upregulation of the ACE2/Ang (1-7)/Mas axis attenuates blood pressure elevation in spontaneously hypertensive rats.

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  • Additional Information
    • Source:
      Publisher: Informa Healthcare Country of Publication: England NLM ID: 9305929 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1525-6006 (Electronic) Linking ISSN: 10641963 NLM ISO Abbreviation: Clin Exp Hypertens Subsets: MEDLINE
    • Publication Information:
      Publication: London : Informa Healthcare
      Original Publication: New York, N.Y. : Marcel Dekker, Inc., c1993-
    • Subject Terms:
      Blood Pressure* ; Sympathectomy, Chemical*; Angiotensin I/*metabolism ; Peptide Fragments/*metabolism ; Peptidyl-Dipeptidase A/*metabolism ; Proto-Oncogene Proteins/*metabolism ; Receptors, G-Protein-Coupled/*metabolism; Angiotensin-Converting Enzyme 2 ; Animals ; Hypertension/physiopathology ; Male ; NADPH Oxidases/blood ; Nitric Oxide/blood ; Nitric Oxide Synthase Type III/blood ; Paraventricular Hypothalamic Nucleus/metabolism ; Proto-Oncogene Mas ; Random Allocation ; Rats ; Rats, Inbred SHR ; Reactive Oxygen Species/blood ; Renal Artery/innervation ; Renin-Angiotensin System ; Up-Regulation
    • Abstract:
      Objective: Evidence has shown that the ACE2/Ang (1-7)/Mas axis plays an important role in the control of hypertension. Thus, we hypothesized that chemical renal denervation (RDN) could reduce blood pressure by regulating the ACE2/Ang (1-7)/Mas axis in spontaneously hypertensive rats.
      Methods: Twelve rats were randomly divided into sham group and chemical RDN group. All the rats were sacrificed 4 weeks later. Plasma samples were collected to measure the renin-angiotensin system (RAS) activities and reactive oxygen species levels by radioimmunoassay, chromatometry and ELISA. Paraventricular nucleus (PVN) tissues were collected to examine the expression of the components of the ACE2/Ang (1-7)/Mas axis by western bolt and immunofluorescence.
      Results: The systolic blood pressure (169.33 ± 7.50 vs 182.67 ± 7.00 mmHg, p < .05) and the diastolic blood pressure (97.50 ± 4.68 vs 109.33 ± 4.41 mmHg, p < .05) in the RDN group were obviously lower than the baseline levels, whereas the opposite results were observed in the sham group. The RDN group exhibited a significant reduction in the plasma ROS (91.59 ± 13.12 vs 72.34 ± 11.76 U/ml, p < .05) and NADPH oxidase (171.86 ± 1.14 vs 175.75 ± 1.74 nmol/ml, p < .001) compared with the sham group, while the plasma eNOS (3.47 ± 0.42 vs 2.49 ± 0.51 U/ml, p < .05) and NO (55.92 ± 8.10 vs 43.53 ± 4.58 μmol/L, p < .05) were increased. The expression of the components of the ACE2/Ang (1-7)/Mas axis was upregulated while the expression of the components of the ACE/Ang II/AT1 R axis was downregulated in the plasma and PVN in the RDN group.
      Conclusion: Our findings suggested that the reduction in blood pressure was regulated by chemical RDN-induced upregulation of the components of the ACE2/Ang (1-7)/Mas axis.
    • Contributed Indexing:
      Keywords: ACE2/Ang (1-7)/Mas; Renal denervation; hypertension
    • Accession Number:
      0 (Peptide Fragments)
      0 (Proto-Oncogene Mas)
      0 (Proto-Oncogene Proteins)
      0 (Reactive Oxygen Species)
      0 (Receptors, G-Protein-Coupled)
      31C4KY9ESH (Nitric Oxide)
      9041-90-1 (Angiotensin I)
      EC 1.14.13.39 (Nitric Oxide Synthase Type III)
      EC 1.14.13.39 (Nos3 protein, rat)
      EC 1.6.3.- (NADPH Oxidases)
      EC 3.4.15.1 (Peptidyl-Dipeptidase A)
      EC 3.4.17.23 (Ace2 protein, rat)
      EC 3.4.17.23 (Angiotensin-Converting Enzyme 2)
      IJ3FUK8MOF (angiotensin I (1-7))
    • Publication Date:
      Date Created: 20200602 Date Completed: 20201117 Latest Revision: 20211204
    • Publication Date:
      20231215
    • Accession Number:
      10.1080/10641963.2020.1772812
    • Accession Number:
      32476477