Leukotriene C4 metabolism during its action on glucose and lactate balance and flow in perfused rat liver.

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  • Author(s): Iwai M;Iwai M; Hagmann W; Keppler D; Jungermann K
  • Source:
    Biological chemistry Hoppe-Seyler [Biol Chem Hoppe Seyler] 1988 Oct; Vol. 369 (10), pp. 1131-6.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Walter De Gruyter Country of Publication: Germany NLM ID: 8503054 Publication Model: Print Cited Medium: Print ISSN: 0177-3593 (Print) Linking ISSN: 01773593 NLM ISO Abbreviation: Biol Chem Hoppe Seyler Subsets: MEDLINE
    • Publication Information:
      Publication: Berlin : Walter De Gruyter
      Original Publication: Berlin ; New York : W. de Gruyter, c1985-c1996.
    • Subject Terms:
      Glucose/*metabolism ; Lactates/*metabolism ; Liver/*metabolism ; SRS-A/*metabolism; Animals ; Bile/metabolism ; Cell Communication ; Liver/drug effects ; Male ; Perfusion ; Rats ; Rats, Inbred Strains ; Reference Values ; SRS-A/pharmacology ; Tritium
    • Abstract:
      Rat livers were perfused in a non-recirculating mode at constant pressure via the portal vein with media containing 5 mM glucose, 2 mM lactate, and 0.2 mM pyruvate. [3H]LTC4 was infused for a period of 5 min to a final concentration of 20 nM; it increased glucose and lactate output and reduced perfusion flow. 1) Leukotriene radioactivity was recovered 10 min after the onset of [3H]LTC4 infusion to about 40% in the effluent, to 20% in the bile, and to 40% in the liver. 2) Radioactivity in the effluent increased to a maximum 4-5 min after the onset and decreased again to essentially zero 3 min after completion of [3H]LTC4 infusion. [3H]LTC4 and [3H]LTD4 were the major labeled components in the effluent accounting for 45% and 38%, respectively, of the effluent radioactivity. 3) [3H]LTC4 and [3H]LTD4 were also the major components in bile; they accounted for 50% and 30%, respectively, of the radioactivity excreted, while more polar [3H]leukotriene metabolites accounted for the remainder. 4) In the liver, [3H]LTC4 and [3H]LTD4 were the major and [3H]LTE4, N-acetyl-[3H]LTE4 as well as omega-hydroxy-N-acetyl-[3H]LTE4 and omega-carboxy-N-acetyl-[3H]LTE4 were minor components detected 5 min after completion of [3H]LTC4 infusion. It is concluded from the present findings that during a 5 min infusion period about one third each of the infused LTC4 remained unchanged, was converted to LTD4, and was further degraded to LTE4 and polar metabolites including omega-oxidation products of N-acetyl-LTE4.(ABSTRACT TRUNCATED AT 250 WORDS)
    • Accession Number:
      0 (Lactates)
      0 (SRS-A)
      10028-17-8 (Tritium)
      IY9XDZ35W2 (Glucose)
    • Publication Date:
      Date Created: 19881001 Date Completed: 19890530 Latest Revision: 20190919
    • Publication Date:
      20221213
    • Accession Number:
      10.1515/bchm3.1988.369.2.1131
    • Accession Number:
      3242543