Localization of the sinoatrial and atrioventricular nodal region in neonatal and juvenile ovine hearts.

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  • Additional Information
    • Source:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
    • Publication Information:
      Original Publication: San Francisco, CA : Public Library of Science
    • Subject Terms:
      Atrioventricular Node/*anatomy & histology ; Sheep/*anatomy & histology ; Sinoatrial Node/*anatomy & histology; Animals
    • Abstract:
      Localization of the components of the cardiac conduction system (CCS) is essential for many therapeutic procedures in cardiac surgery and interventional cardiology. While histological studies provided fundamental insights into CCS localization, this information is incomplete and difficult to translate to aid in intraprocedural localization. To advance our understanding of CCS localization, we set out to establish a framework for quantifying nodal region morphology. Using this framework, we quantitatively analyzed the sinoatrial node (SAN) and atrioventricular node (AVN) in ovine with postmenstrual age ranging from 4.4 to 58.3 months. In particular, we studied the SAN and AVN in relation to the epicardial and endocardial surfaces, respectively. Using anatomical landmarks, we excised the nodes and adjacent tissues, sectioned those at a thickness of 4 μm at 100 μm intervals, and applied Masson's trichrome stain to the sections. These sections were then imaged, segmented to identify nodal tissue, and analyzed to quantify nodal depth and superficial tissue composition. The minimal SAN depth ranged between 20 and 926 μm. AVN minimal depth ranged between 59 and 1192 μm in the AVN extension region, 49 and 980 μm for the compact node, and 148 and 888 μm for the transition to His Bundle region. Using a logarithmic regression model, we found that minimal depth increased logarithmically with age for the AVN (R2 = 0.818, P = 0.002). Also, the myocardial overlay of the AVN was heterogeneous within different regions and decreased with increasing age. Age associated alterations of SAN minimal depth were insignificant. Our study presents examples of characteristic tissue patterns superficial to the AVN and within the SAN. We suggest that the presented framework provides quantitative information for CCS localization. Our studies indicate that procedural methods and localization approaches in regions near the AVN should account for the age of patients in cardiac surgery and interventional cardiology.
      Competing Interests: The authors have declared that no competing interests exist.
    • References:
      Am J Cardiol. 1975 Jan;35(1):23-9. (PMID: 122784)
      Am Heart J. 1996 Mar;131(3):499-507. (PMID: 8604629)
      Sci Rep. 2017 Aug 3;7(1):7188. (PMID: 28775383)
      Am J Cardiol. 1977 Dec;40(6):965-86. (PMID: 930844)
      Ann N Y Acad Sci. 1965 Sep 8;127(1):19-33. (PMID: 5217260)
      PLoS One. 2012;7(4):e35299. (PMID: 22509404)
      Chest. 1970 Oct;58(4):342-4. (PMID: 5506627)
      Circulation. 1963 Jul;28:82-8. (PMID: 13985178)
      J Cardiothorac Surg. 2010 Sep 28;5:74. (PMID: 20920167)
      JAMA. 1968 Oct 14;206(3):645-6. (PMID: 5695590)
      Pediatr Med Chir. 1998 May-Jun;20(3):181-6. (PMID: 9744009)
      Anat Rec (Hoboken). 2011 Jun;294(6):970-9. (PMID: 21538926)
      Circulation. 2006 Jun 13;113(23):2775-81. (PMID: 16769927)
      Development. 2016 Jan 15;143(2):197-210. (PMID: 26786210)
      Circulation. 2009 Mar 31;119(12):1562-75. (PMID: 19289639)
      Clin Anat. 2009 Jan;22(1):99-113. (PMID: 18773472)
      Heart Rhythm. 2014 Mar;11(3):529-30. (PMID: 23354077)
      World J Pediatr Congenit Heart Surg. 2015 Apr;6(2):239-49. (PMID: 25870343)
      Am Heart J. 1995 Apr;129(4):799-808. (PMID: 7900634)
      J Anat Physiol. 1907 Apr;41(Pt 3):172-89. (PMID: 17232727)
      Circ Res. 1993 Sep;73(3):482-91. (PMID: 8394224)
      N Engl J Med. 1961 Nov 30;265:1079-83. (PMID: 13868149)
      Prog Biophys Mol Biol. 2016 Jan;120(1-3):164-78. (PMID: 26743207)
      Br Heart J. 1971 Sep;33(5):639-42. (PMID: 5115008)
      J Morphol. 2017 Jul;278(7):975-986. (PMID: 28444887)
      Pacing Clin Electrophysiol. 2010 Jun 1;33(6):754-62. (PMID: 20180918)
      Br Heart J. 1979 Sep;42(3):339-44. (PMID: 508456)
      Am J Cardiol. 1961 May;7:681-9. (PMID: 13738797)
      Semin Thorac Cardiovasc Surg. 2019 Autumn;31(3):537-546. (PMID: 30738149)
      Circulation. 2011 Mar 1;123(8):904-15. (PMID: 21357845)
      Circulation. 2000 Feb 29;101(8):869-77. (PMID: 10694526)
      Sci Rep. 2017 Mar 23;7(1):344. (PMID: 28336939)
      Pharmacol Ther. 2013 Aug;139(2):260-88. (PMID: 23612425)
      Arch Intern Med. 1976 Apr;136(4):406-8. (PMID: 1267548)
      Circ Res. 1993 Sep;73(3):473-81. (PMID: 8394223)
      Nat Methods. 2012 Jun 28;9(7):676-82. (PMID: 22743772)
      J Am Coll Cardiol. 2000 Jul;36(1):255-61. (PMID: 10898443)
      Circ Cardiovasc Imaging. 2013 Sep;6(5):739-46. (PMID: 23811748)
      Ann Anat. 2011 Feb 20;193(1):1-12. (PMID: 21145220)
      Heart Rhythm. 2006 May;3(5):601-4. (PMID: 16648069)
      Circ J. 2003 Feb;67(2):107-11. (PMID: 12547989)
      Am J Cardiol. 1972 Mar;29(3):344-50. (PMID: 5060806)
      World J Pediatr Congenit Heart Surg. 2016 Nov;7(6):700-705. (PMID: 27834761)
      Circulation. 1976 Apr;53(4):609-21. (PMID: 1253382)
      J Am Coll Cardiol. 1998 Oct;32(4):1040-7. (PMID: 9768730)
      Heart. 2005 Feb;91(2):189-94. (PMID: 15657230)
      Br Heart J. 1984 Jun;51(6):618-21. (PMID: 6610435)
      Eur J Cardiothorac Surg. 2018 Apr 1;53(suppl_1):i19-i25. (PMID: 29590383)
      Tohoku J Exp Med. 2013;229(1):67-73. (PMID: 23269205)
      J Interv Card Electrophysiol. 2016 Jun;46(1):3-8. (PMID: 26319648)
      J Cardiovasc Dev Dis. 2017;4(2):. (PMID: 29098150)
      Dev Genet. 1999;24(1-2):82-90. (PMID: 10079513)
      A A Case Rep. 2015 Aug 1;5(3):33-5. (PMID: 26230304)
      J Interv Card Electrophysiol. 2005 Jul;13(2):95-105. (PMID: 16133836)
      Cardiol Young. 2006 Oct;16(5):437-54. (PMID: 16984696)
      Pacing Clin Electrophysiol. 2009 Jan;32(1):7-12. (PMID: 19140907)
      Physiol Rev. 1988 Apr;68(2):608-47. (PMID: 2451833)
      Circ Res. 1991 Aug;69(2):429-37. (PMID: 1860183)
      Circ Res. 1969 Mar;24(3):457-74. (PMID: 5766521)
      FEBS Lett. 1987 May 25;216(1):35-9. (PMID: 3108033)
    • Grant Information:
      R01 HL135077 United States HL NHLBI NIH HHS; R56 HL128813 United States HL NHLBI NIH HHS
    • Publication Date:
      Date Created: 20200508 Date Completed: 20200909 Latest Revision: 20200909
    • Publication Date:
      20231215
    • Accession Number:
      PMC7205220
    • Accession Number:
      10.1371/journal.pone.0232618
    • Accession Number:
      32379798