Lycium barbarum polysaccharides ameliorate intestinal barrier dysfunction and inflammation through the MLCK-MLC signaling pathway in Caco-2 cells.

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  • Author(s): Li W;Li W; Gao M; Gao M; Han T; Han T
  • Source:
    Food & function [Food Funct] 2020 Apr 30; Vol. 11 (4), pp. 3741-3748.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101549033 Publication Model: Print Cited Medium: Internet ISSN: 2042-650X (Electronic) Linking ISSN: 20426496 NLM ISO Abbreviation: Food Funct Subsets: MEDLINE
    • Publication Information:
      Original Publication: Cambridge : Royal Society of Chemistry
    • Subject Terms:
      Caco-2 Cells/*drug effects ; Drugs, Chinese Herbal/*pharmacology; Caco-2 Cells/metabolism ; Humans ; Intestinal Mucosa/metabolism ; Myosin Light Chains/metabolism ; Myosin-Light-Chain Kinase/metabolism ; Signal Transduction
    • Abstract:
      Impairment of the intestinal barrier often occurs in inflammatory bowel diseases, and pro-inflammatory factors play a vital role in the pathogenesis of intestinal diseases. In our study, the potential protective effects of Lycium barbarum polysaccharides (LBP) against intestinal barrier dysfunction evoked by pro-inflammatory factors and its anti-inflammatory effects were investigated. Caco-2 cells were stimulated with or without tumor necrosis factor (TNF)-α in the presence or absence of LBP. Our findings showed that LBP assuaged the increase of paracellular permeability and the decrease of transepithelial electrical resistance (TER) in Caco-2 cells. In addition, LBP also prevented the secretion of pro-inflammatory markers (IL-8, IL-6, ICAM-1 and MCP-1) in TNF-α-challenged Caco-2 cells. Moreover, LBP inhibited the overexpression of tight junction (TJ) proteins (claudin-1, ZO-3, and occludin) and the increase of MLCK, pMLC, p-IκBα and NFκBp65 protein expression evoked by TNF-α was suppressed by LBP pre-incubation. This finding indicated that LBP improve TNF-α-evoked intestinal barrier dysfunction via suppressing the MLCK-MLC signaling pathway mediated by NFκB.
    • Accession Number:
      0 (Drugs, Chinese Herbal)
      0 (Myosin Light Chains)
      0 (lycium barbarum polysaccharide)
      EC 2.7.11.18 (Myosin-Light-Chain Kinase)
    • Publication Date:
      Date Created: 20200422 Date Completed: 20210224 Latest Revision: 20210224
    • Publication Date:
      20240829
    • Accession Number:
      10.1039/d0fo00030b
    • Accession Number:
      32314770