Highly efficient genome editing in N. gerenzanensis using an inducible CRISPR/Cas9-RecA system.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Kluwer Academic Publishers Country of Publication: Netherlands NLM ID: 8008051 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-6776 (Electronic) Linking ISSN: 01415492 NLM ISO Abbreviation: Biotechnol Lett Subsets: MEDLINE
    • Publication Information:
      Publication: 1999- : Dordrecht : Kluwer Academic Publishers
      Original Publication: [Kew, Eng., Science and Technology Letters]
    • Subject Terms:
      Actinobacteria/*genetics ; CRISPR-Cas Systems/*genetics ; Gene Editing/*methods ; Rec A Recombinases/*genetics; Actinomyces/genetics ; Bacterial Proteins/genetics ; Escherichia coli/genetics ; Mutation/genetics
    • Abstract:
      Objective: To develop an inducible CRISPR/Cas9-Recombinase A (RecA) system to manipulate genes in Nonomuraea gerenzanensis effectively.
      Results: Compared with traditional homologous recombination, the inducible CRISPR/Cas9 system achieved 68.8% editing efficiency, whereas, with both the inducible Cas9 and the overexpressed RecA, the efficiency of the combined genome editing system reached 100%. The dbv23-deleted mutant obtained by the inducible CRISPR/Cas9-RecA system was confirmed to produce more A40926 with an approximate yield of 200 mg L -1 than that of around 150 mg L -1 produced by the wild-type strain.
      Conclusions: This inducible CRISPR/Cas9-RecA system was successfully constructed and can be utilized as an efficient genome editing tool for Actinomyces able to shorten editing time simultaneously.
    • Grant Information:
      ZR2015CL001 Natural Science Foundation of Shandong Province
    • Contributed Indexing:
      Keywords: A40926; CRISPR/Cas9; Genome editing; N. gerenzanensis; RecA
    • Accession Number:
      0 (Bacterial Proteins)
      EC 2.7.7.- (Rec A Recombinases)
    • Subject Terms:
      Nonomuraea gerenzanensis
    • Publication Date:
      Date Created: 20200422 Date Completed: 20210224 Latest Revision: 20210224
    • Publication Date:
      20231215
    • Accession Number:
      10.1007/s10529-020-02893-2
    • Accession Number:
      32314149