Challenges in identification of enteroinvasive Escherichia coli and Shigella spp. in Lebanon.

Publisher: Akademiai Kiado Country of Publication: Hungary NLM ID: 9434021 Publication Model: Electronic Cited Medium: Internet ISSN: 1588-2640 (Electronic) Linking ISSN: 12178950 NLM ISO Abbreviation: Acta Microbiol Immunol Hung Subsets: MEDLINE

Publication: Budapest : Akademiai Kiado
Original Publication: Budapest : Akadémiai Kiadó, 1994-

Dysentery, Bacillary/*diagnosis ; Enteropathogenic Escherichia coli/*isolation & purification ; Molecular Typing/*methods ; Shigella/*isolation & purification; Antigens, Bacterial/genetics ; Bacterial Proteins/genetics ; DNA Gyrase/genetics ; Drug Resistance, Multiple, Bacterial/genetics ; Dysentery, Bacillary/microbiology ; Enteropathogenic Escherichia coli/classification ; Enteropathogenic Escherichia coli/genetics ; Escherichia coli Proteins/genetics ; Humans ; Lebanon ; Microbial Sensitivity Tests ; Monosaccharide Transport Proteins/genetics ; Shiga Toxin/metabolism ; Shigella/classification ; Shigella/genetics ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Symporters/genetics

This study aimed to evaluate the routine identification tools available in Lebanon for differentiation of Escherichia coli and Shigella spp. The identification of 43 isolates defined as Shigella spp. by Api 20E was accessed using MALDI-TOF, serological testing, duplex PCR targeting ipaH (present in Shigella spp. and enteroinvasive E. coli "EIEC") and lacY (found in E. coli including EIEC but not Shigella spp.) as well as gyrB gene sequencing. Antibiotic susceptibility was investigated as well as Shiga-toxin production. All isolates were identified as E. coli by MALDI-TOF while the PCR showed a disparate group of 26 EIEC, 11 Shigella spp., 5 E. coli and 1 inactive E. coli. However, the sequencing of gyrB gene, which was recently described as a suitable marker for distinguishing E. coli and Shigella spp., identified all isolates as E. coli. Antibiotic resistance was noticeable against ß-lactams, rifampicin, trimethoprim-sulfamethoxazole, gentamicin, and ciprofloxacin. The most common variants of beta-lactamase genes were blaTEM-1, blaCTX-M-15, and blaCTX-M-3. A great discordance between the used methods in identification was revealed herein. An accurate identification technique able to distinguish E. coli from Shigella spp. in routine laboratories is a pressing need in order to select the appropriate treatment and assess the epidemiology of these bacteria.

Keywords: EIEC; Escherichia coli; Lebanon; Shigella spp.; identification; molecular techniques; phenotypic techniques

0 (Antigens, Bacterial)
0 (Bacterial Proteins)
0 (Escherichia coli Proteins)
0 (LacY protein, E coli)
0 (Monosaccharide Transport Proteins)
0 (Symporters)
0 (ipaH protein, E coli)
0 (ipaH protein, Shigella flexneri)
75757-64-1 (Shiga Toxin)
EC 5.99.1.3 (DNA Gyrase)

Date Created: 20200401 Date Completed: 20210722 Latest Revision: 20210722

20231215

10.1556/030.2020.01102

32223306