Drosophila-associated bacteria differentially shape the nutritional requirements of their host during juvenile growth.

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  • Additional Information
    • Source:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101183755 Publication Model: eCollection Cited Medium: Internet ISSN: 1545-7885 (Electronic) Linking ISSN: 15449173 NLM ISO Abbreviation: PLoS Biol Subsets: MEDLINE
    • Publication Information:
      Original Publication: San Francisco, CA : Public Library of Science, [2003]-
    • Subject Terms:
      Acetobacter/*physiology ; Drosophila melanogaster/*microbiology ; Drosophila melanogaster/*physiology ; Lactobacillus/*physiology ; Nutritional Requirements/*physiology; Acetobacter/genetics ; Acetobacter/metabolism ; Amino Acids/metabolism ; Animal Nutritional Physiological Phenomena ; Animals ; Drosophila melanogaster/growth & development ; Drosophila melanogaster/metabolism ; Gastrointestinal Microbiome ; Host Microbial Interactions ; Lactobacillus/genetics ; Lactobacillus/metabolism ; Larva/growth & development ; Larva/metabolism ; Larva/microbiology ; Larva/physiology ; Metabolic Networks and Pathways ; Micronutrients/metabolism ; Species Specificity
    • Abstract:
      The interplay between nutrition and the microbial communities colonizing the gastrointestinal tract (i.e., gut microbiota) determines juvenile growth trajectory. Nutritional deficiencies trigger developmental delays, and an immature gut microbiota is a hallmark of pathologies related to childhood undernutrition. However, how host-associated bacteria modulate the impact of nutrition on juvenile growth remains elusive. Here, using gnotobiotic Drosophila melanogaster larvae independently associated with Acetobacter pomorumWJL (ApWJL) and Lactobacillus plantarumNC8 (LpNC8), 2 model Drosophila-associated bacteria, we performed a large-scale, systematic nutritional screen based on larval growth in 40 different and precisely controlled nutritional environments. We combined these results with genome-based metabolic network reconstruction to define the biosynthetic capacities of Drosophila germ-free (GF) larvae and its 2 bacterial partners. We first established that ApWJL and LpNC8 differentially fulfill the nutritional requirements of the ex-GF larvae and parsed such difference down to individual amino acids, vitamins, other micronutrients, and trace metals. We found that Drosophila-associated bacteria not only fortify the host's diet with essential nutrients but, in specific instances, functionally compensate for host auxotrophies by either providing a metabolic intermediate or nutrient derivative to the host or by uptaking, concentrating, and delivering contaminant traces of micronutrients. Our systematic work reveals that beyond the molecular dialogue engaged between the host and its bacterial partners, Drosophila and its associated bacteria establish an integrated nutritional network relying on nutrient provision and utilization.
      Competing Interests: The authors have declared that no competing interests exist.
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    • Accession Number:
      0 (Amino Acids)
      0 (Micronutrients)
    • Publication Date:
      Date Created: 20200321 Date Completed: 20200626 Latest Revision: 20200626
    • Publication Date:
      20231215
    • Accession Number:
      PMC7112240
    • Accession Number:
      10.1371/journal.pbio.3000681
    • Accession Number:
      32196485