BDNF impact on synaptic dynamics: extra or intracellular long-term release differently regulates cultured hippocampal synapses.

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  • Additional Information
    • Source:
      Publisher: BioMed Central Country of Publication: England NLM ID: 101468876 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-6606 (Electronic) Linking ISSN: 17566606 NLM ISO Abbreviation: Mol Brain Subsets: MEDLINE
    • Publication Information:
      Original Publication: London : BioMed Central
    • Subject Terms:
      Brain-Derived Neurotrophic Factor/*pharmacology ; Extracellular Space/*metabolism ; Hippocampus/*metabolism ; Intracellular Space/*metabolism ; Synapses/*metabolism; Animals ; Cells, Cultured ; Glutamic Acid/metabolism ; Neurons/drug effects ; Neurons/metabolism ; Rats ; Receptor, trkB/metabolism ; Synapses/drug effects ; Vesicular Inhibitory Amino Acid Transport Proteins/metabolism
    • Abstract:
      Brain Derived Neurotrophic Factor (BDNF) signalling contributes to the formation, maturation and plasticity of Central Nervous System (CNS) synapses. Acute exposure of cultured brain circuits to BDNF leads to up-regulation of glutamatergic neuro-transmission, by the accurate tuning of pre and post synaptic features, leading to structural and functional synaptic changes. Chronic BDNF treatment has been comparatively less investigated, besides it may represent a therapeutic option to obtain rescue of post-injury alterations of synaptic networks. In this study, we used a paradigm of BDNF long-term (4 days) incubation to assess in hippocampal neurons in culture, the ability of such a treatment to alter synapses. By patch clamp recordings we describe the augmented function of excitatory neurotransmission and we further explore by live imaging the presynaptic changes brought about by long-term BDNF. In our study, exogenous long-term BDNF exposure of post-natal neurons did not affect inhibitory neurotransmission. We further compare, by genetic manipulations of cultured neurons and BDNF release, intracellular overexpression of this neurotrophin at the same developmental age. We describe for the first-time differences in synaptic modulation by BDNF with respect to exogenous or intracellular release paradigms. Such a finding holds the potential of influencing the design of future therapeutic strategies.
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    • Contributed Indexing:
      Keywords: Hippocampal network; Miniature synaptic current; Neurotrophin; Patch-clamp; Synaptic activity
    • Accession Number:
      0 (Brain-Derived Neurotrophic Factor)
      0 (Slc32a1 protein, rat)
      0 (Vesicular Inhibitory Amino Acid Transport Proteins)
      3KX376GY7L (Glutamic Acid)
      EC 2.7.10.1 (Receptor, trkB)
    • Publication Date:
      Date Created: 20200319 Date Completed: 20210203 Latest Revision: 20210203
    • Publication Date:
      20221213
    • Accession Number:
      PMC7079446
    • Accession Number:
      10.1186/s13041-020-00582-9
    • Accession Number:
      32183860