Upregulation of Fucosyltransferase 3, 8 and protein O-Fucosyltransferase 1, 2 genes in esophageal cancer stem-like cells (CSLCs).

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  • Additional Information
    • Source:
      Publisher: Springer Country of Publication: United States NLM ID: 8603310 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-4986 (Electronic) Linking ISSN: 02820080 NLM ISO Abbreviation: Glycoconj J Subsets: MEDLINE
    • Publication Information:
      Publication: <2008->: Norwell, MA : Springer
      Original Publication: [Lund : The Journal, c1984-
    • Subject Terms:
      Esophageal Neoplasms*/enzymology ; Esophageal Neoplasms*/genetics ; Esophageal Squamous Cell Carcinoma*/enzymology ; Esophageal Squamous Cell Carcinoma*/genetics ; Fucosyltransferases*/genetics ; Fucosyltransferases*/metabolism ; Neoplastic Stem Cells*/enzymology; Cell Line, Tumor ; Humans ; Up-Regulation
    • Abstract:
      Recently, studies have shown that Fucosylation plays an important role in the invasion and metastatic process of CSLCs. Understanding the expression pattern of fucosyltransferase (FUT) genes may help to suggest better-targeted therapy strategies for esophageal squamous cell carcinoma (ESCC). The study aimed to address the expression pattern of FUT gene variants in esophageal CSLCs and parental adherent cells. Sphere formation method was used to enrich CSLCs. Expression of FUT genes was examined in tumor sphere and parental adherent cells using the RT-PCR method and then relative expression of detected variants was performed by the Real-Time PCR method in both groups. The detected FUTs, also, were assessed in fresh ESCC tumors and the matched healthy controls. Analysis of The cell surface carbohydrate Lewis x (LeX, CD15) was performed by flow cytometry. Molecular analysis showed that the expression of FUT 3, 8 and POFUT1, 2 genes in tumorsphere were significantly higher than parental adherent cells. Analysis of fresh ESCC tumor tissues and the matched healthy controls showed that FUT8 and POFUT1, 2 genes in contrast to FUT 3 have higher expression in tumor tissues than controls. Flow cytometric analyses revealed that tumorsphere and their parent cells do not differ significantly in Lewis x surface marker. The present study showed that FUT 3, 8 and POFUT1, 2 genes upregulated in esophageal CSLCs in comparison to adherent cells. Understanding the expression pattern of FUT gene variants may help to suggest better-targeted therapy strategies for ESCC.
    • Grant Information:
      970308034 Golestan University of Medical Sciences
    • Contributed Indexing:
      Keywords: Cancer stem-like cells (CSLCs); ESCC cancer; Fucosyltransferase genes; Lewis x
    • Accession Number:
      EC 2.4.1.- (Fucosyltransferases)
      EC 2.4.1.221 (POFUT2 protein, human)
      EC 2.4.1.221 (polypeptide fucosyltransferase)
      EC 2.4.1.65 (3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase)
      EC 2.4.1.68 (Glycoprotein 6-alpha-L-fucosyltransferase)
    • Publication Date:
      Date Created: 20200312 Date Completed: 20220530 Latest Revision: 20220601
    • Publication Date:
      20231215
    • Accession Number:
      10.1007/s10719-020-09917-z
    • Accession Number:
      32157457