Metabolic alterations provide insights into Stylosanthes roots responding to phosphorus deficiency.

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  • Additional Information
    • Source:
      Publisher: BioMed Central Country of Publication: England NLM ID: 100967807 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2229 (Electronic) Linking ISSN: 14712229 NLM ISO Abbreviation: BMC Plant Biol Subsets: MEDLINE
    • Publication Information:
      Original Publication: London : BioMed Central, [2001-
    • Subject Terms:
      Metabolome*; Fabaceae/*metabolism ; Phosphorus/*deficiency ; Phosphorus Compounds/*metabolism ; Plant Roots/*metabolism; Fabaceae/genetics ; Gene Expression ; Genes, Plant ; Soil/chemistry
    • Abstract:
      Background: Phosphorus (P) deficiency is one of the major constraints limiting plant growth, especially in acid soils. Stylosanthes (stylo) is a pioneer tropical legume with excellent adaptability to low P stress, but its underlying mechanisms remain largely unknown.
      Results: In this study, the physiological, molecular and metabolic changes in stylo responding to phosphate (Pi) starvation were investigated. Under low P condition, the growth of stylo root was enhanced, which was attributed to the up-regulation of expansin genes participating in root growth. Metabolic profiling analysis showed that a total of 256 metabolites with differential accumulations were identified in stylo roots response to P deficiency, which mainly included flavonoids, sugars, nucleotides, amino acids, phenylpropanoids and phenylamides. P deficiency led to significant reduction in the accumulation of phosphorylated metabolites (e.g., P-containing sugars, nucleotides and cholines), suggesting that internal P utilization was enhanced in stylo roots subjected to low P stress. However, flavonoid metabolites, such as kaempferol, daidzein and their glycoside derivatives, were increased in P-deficient stylo roots. Furthermore, the qRT-PCR analysis showed that a set of genes involved in flavonoids synthesis were found to be up-regulated by Pi starvation in stylo roots. In addition, the abundances of phenolic acids and phenylamides were significantly increased in stylo roots during P deficiency. The increased accumulation of the metabolites in stylo roots, such as flavonoids, phenolic acids and phenylamides, might facilitate P solubilization and cooperate with beneficial microorganisms in rhizosphere, and thus contributing to P acquisition and utilization in stylo.
      Conclusions: These results suggest that stylo plants cope with P deficiency by modulating root morphology, scavenging internal Pi from phosphorylated metabolites and increasing accumulation of flavonoids, phenolic acids and phenylamides. This study provides valuable insights into the complex responses and adaptive mechanisms of stylo roots to P deficiency.
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    • Grant Information:
      318QN265 Natural Science Foundation of Hainan Province; 31701492, 31861143013, 31672483 National Natural Science Foundation of China; CARS-34, CARS-22-Z11 Modern Agro-industry Technology Research System; 1630032018004 Central Public-interest Scientific Institution Basal Research Fund for CATAS
    • Contributed Indexing:
      Keywords: Expansin; Flavonoid; Metabolome; Phosphorus deficiency; Root morphology; Stylosanthes
    • Accession Number:
      0 (Phosphorus Compounds)
      0 (Soil)
      27YLU75U4W (Phosphorus)
    • Publication Date:
      Date Created: 20200224 Date Completed: 20201027 Latest Revision: 20240328
    • Publication Date:
      20240329
    • Accession Number:
      PMC7036231
    • Accession Number:
      10.1186/s12870-020-2283-z
    • Accession Number:
      32087672