FIGLA, LHX8 and SOHLH1 transcription factor networks regulate mouse oocyte growth and differentiation.

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  • Author(s): Wang Z;Wang Z; Liu CY; Liu CY; Zhao Y; Zhao Y; Dean J; Dean J
  • Source:
    Nucleic acids research [Nucleic Acids Res] 2020 Apr 17; Vol. 48 (7), pp. 3525-3541.
  • Publication Type:
    Journal Article; Research Support, N.I.H., Intramural
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE
    • Publication Information:
      Publication: 1992- : Oxford : Oxford University Press
      Original Publication: London, Information Retrieval ltd.
    • Subject Terms:
      Gene Regulatory Networks*; Basic Helix-Loop-Helix Transcription Factors/*metabolism ; LIM-Homeodomain Proteins/*metabolism ; Oocytes/*metabolism ; Oogenesis/*genetics ; Transcription Factors/*metabolism; Animals ; Apoptosis ; Basic Helix-Loop-Helix Transcription Factors/genetics ; Cell Proliferation/genetics ; DNA Damage ; Female ; Gene Expression Regulation ; HEK293 Cells ; Humans ; LIM-Homeodomain Proteins/genetics ; Meiosis/genetics ; Mice ; Oocytes/cytology ; Ovary/metabolism ; Transcription Factors/genetics
    • Abstract:
      Germ-cell transcription factors control gene networks that regulate oocyte differentiation and primordial follicle formation during early, postnatal mouse oogenesis. Taking advantage of gene-edited mice lacking transcription factors expressed in female germ cells, we analyzed global gene expression profiles in perinatal ovaries from wildtype, FiglaNull, Lhx8Null and Sohlh1Null mice. Figla deficiency dysregulates expression of meiosis-related genes (e.g. Sycp3, Rad51, Ybx2) and a variety of genes (e.g. Nobox, Lhx8, Taf4b, Sohlh1, Sohlh2, Gdf9) associated with oocyte growth and differentiation. The absence of FIGLA significantly impedes meiotic progression, causes DNA damage and results in oocyte apoptosis. Moreover, we find that FIGLA and other transcriptional regulator proteins (e.g. NOBOX, LHX8, SOHLH1, SOHLH2) are co-expressed in the same subset of germ cells in perinatal ovaries and Figla ablation dramatically disrupts KIT, NOBOX, LHX8, SOHLH1 and SOHLH2 abundance. In addition, not only do FIGLA, LHX8 and SOHLH1 cross-regulate each other, they also cooperate by direct interaction with each during early oocyte development and share downstream gene targets. Thus, our findings substantiate a major role for FIGLA, LHX8 and SOHLH1 as multifunctional regulators of networks necessary for oocyte maintenance and differentiation during early folliculogenesis.
      (Published by Oxford University Press on behalf of Nucleic Acids Research 2020.)
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    • Accession Number:
      0 (Basic Helix-Loop-Helix Transcription Factors)
      0 (Figla protein, mouse)
      0 (LIM homeobox protein 8)
      0 (LIM-Homeodomain Proteins)
      0 (Sohlh1 protein, mouse)
      0 (Transcription Factors)
    • Publication Date:
      Date Created: 20200223 Date Completed: 20200706 Latest Revision: 20200706
    • Publication Date:
      20240829
    • Accession Number:
      PMC7144910
    • Accession Number:
      10.1093/nar/gkaa101
    • Accession Number:
      32086523