Identification and Molecular Characterization of a Pellino Protein in Kuruma Prawn ( Marsupenaeus Japonicus ) in Response to White Spot Syndrome Virus and Vibrio Parahaemolyticus Infection.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Arthropod Proteins/*genetics ; Penaeidae/*genetics ; Ubiquitin-Protein Ligases/*genetics ; Vibrio Infections/*genetics; Amino Acid Sequence/genetics ; Animals ; Arthropod Proteins/isolation & purification ; China ; Gene Expression Profiling/methods ; Hemocytes/microbiology ; Hemocytes/virology ; Humans ; Immunity, Innate/genetics ; Penaeidae/microbiology ; Penaeidae/virology ; Toll-Like Receptors/genetics ; Transcriptional Activation/genetics ; Vibrio Infections/microbiology ; Vibrio parahaemolyticus/pathogenicity ; White spot syndrome virus 1/genetics ; White spot syndrome virus 1/pathogenicity

Kuruma prawn, Marsupenaeus japonicus , has the third largest annual yield among shrimp species with vital economic significance in China. White spot syndrome virus (WSSV) is a great threat to the global shrimp farming industry and results in high mortality. Pellino, a highly conserved E3 ubiquitin ligase, has been found to be an important modulator of the Toll-like receptor (TLR) signaling pathways that participate in the innate immune response and ubiquitination. In the present study, the Pellino gene from Marsupenaeus japonicus was identified. A qRT-PCR assay showed the presence of MjPellino in all the tested tissues and revealed that the transcript level of this gene was significantly upregulated in both the gills and hemocytes after challenge with WSSV and Vibrio parahaemolyticus . The function of MjPellino was further verified at the protein level. The results of the three-dimensional modeling and protein-protein docking analyses and a GST pull-down assay revealed that the MjPellino protein was able to bind to the WSSV envelope protein VP26. In addition, the knockdown of MjPellino in vivo significantly decreased the expression of MjAMPs. These results suggest that MjPellino might play an important role in the immune response of kuruma prawn.

Immunol Lett. 2014 Mar-Apr;158(1-2):14-24. (PMID: 24291116)
J Biochem Mol Biol. 2005 Mar 31;38(2):128-50. (PMID: 15826490)
Dev Comp Immunol. 2014 Jun;44(2):341-50. (PMID: 24463313)
J Bioinform Comput Biol. 2016 Jun;14(3):1650011. (PMID: 26932275)
Methods. 2001 Dec;25(4):402-8. (PMID: 11846609)
Acta Microbiol Immunol Hung. 2003;50(4):407-31. (PMID: 14750441)
Nucleic Acids Res. 2014 Jul;42(Web Server issue):W320-4. (PMID: 24753421)
J Virol. 2006 Nov;80(21):10615-23. (PMID: 16928742)
J Biol Chem. 2012 Jul 20;287(30):25686-95. (PMID: 22669975)
Bioinformatics. 2007 Nov 1;23(21):2947-8. (PMID: 17846036)
Fish Shellfish Immunol. 2017 Nov;70:710-719. (PMID: 28943297)
Microbiol Mol Biol Rev. 2003 Jun;67(2):226-37, table of contents. (PMID: 12794191)
Mech Dev. 2010 May-Jun;127(5-6):301-7. (PMID: 20117206)
J Cell Mol Med. 2007 May-Jun;11(3):453-61. (PMID: 17635639)
J Leukoc Biol. 2015 Dec;98(6):963-74. (PMID: 26310831)
Dev Comp Immunol. 2013 Jan-Feb;39(1-2):11-26. (PMID: 22484214)
Fish Shellfish Immunol. 2013 Apr;34(4):1018-24. (PMID: 23416697)
Trends Cell Biol. 2004 Mar;14(3):103-6. (PMID: 15055197)
J Virol. 2007 Jun;81(12):6709-17. (PMID: 17409146)
Microbiol Res. 2019 May;222:43-51. (PMID: 30928029)
Virology. 2005 May 25;336(1):93-9. (PMID: 15866074)
J Gen Virol. 2014 Aug;95(Pt 8):1799-1808. (PMID: 24836670)
Trends Immunol. 2009 Jan;30(1):33-42. (PMID: 19022706)
Proteins. 2003 Jul 1;52(1):80-7. (PMID: 12784371)
J Gen Virol. 2002 Feb;83(Pt 2):471-477. (PMID: 11807241)
J Virol. 2006 Mar;80(6):3021-9. (PMID: 16501111)
PLoS Pathog. 2018 Sep 26;14(9):e1007109. (PMID: 30256850)
Immunol Rev. 2015 Jul;266(1):93-108. (PMID: 26085209)
J Virol. 2008 Dec;82(24):12598-601. (PMID: 18842736)
Virology. 2000 Jan 20;266(2):227-36. (PMID: 10639309)
J Biol Chem. 2003 Mar 28;278(13):10952-6. (PMID: 12496252)
J Mol Biol. 1977 May 25;112(3):535-42. (PMID: 875032)
Front Immunol. 2018 Jul 02;9:1523. (PMID: 30034391)
J Virol. 2004 Oct;78(20):11360-70. (PMID: 15452257)
Mar Environ Res. 2015 Dec;112(Pt A):96-103. (PMID: 26476689)
Virus Res. 2004 Dec;106(2):167-80. (PMID: 15567495)
Comput Appl Biosci. 1997 Aug;13(4):425-30. (PMID: 9283757)
Structure. 2008 Dec 10;16(12):1806-16. (PMID: 19081057)
Cell Death Differ. 2017 Mar;24(3):469-480. (PMID: 28009353)
Antiviral Res. 2013 Sep;99(3):270-80. (PMID: 23773856)
Arch Virol. 2011 Jul;156(7):1125-35. (PMID: 21380712)
Bioinformatics. 2014 Jun 15;30(12):1771-3. (PMID: 24532726)
Dev Comp Immunol. 2014 Feb;42(2):174-85. (PMID: 24012725)
Mech Dev. 1999 Mar;81(1-2):127-38. (PMID: 10330490)
PLoS One. 2011;6(9):e24773. (PMID: 21931849)
Mol Biol Evol. 2016 Jul;33(7):1870-4. (PMID: 27004904)
Methods Mol Biol. 2008;443:365-82. (PMID: 18446297)
J Virol. 2019 Jan 17;93(3):. (PMID: 30404800)
Curr Protoc Bioinformatics. 2006 Oct;Chapter 5:Unit-5.6. (PMID: 18428767)

2019YFD0900803 National Key Research and Development Project; CARS-48 the China Agriculture Research System; 2019B10011 Science and Technology Plan Project of Ningbo

Keywords: Marsupenaeus japonicus; MjPellino; VP26; Vibrio parahaemolyticus; WSSV; immune response; protein-protein docking

0 (Arthropod Proteins)
0 (Toll-Like Receptors)
EC 2.3.2.27 (Ubiquitin-Protein Ligases)

Date Created: 20200220 Date Completed: 20201119 Latest Revision: 20201119

20231215

PMC7072872

10.3390/ijms21041243

32069894