Oral Intake of EPA:DHA 6:1 by Middle-Aged Rats for One Week Improves Age-Related Endothelial Dysfunction in Both the Femoral Artery and Vein: Role of Cyclooxygenases.

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  • Additional Information
    • Source:
      Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
    • Publication Information:
      Original Publication: Basel, Switzerland : MDPI, [2000-
    • Subject Terms:
      Aging/*pathology ; Docosahexaenoic Acids/*administration & dosage ; Eicosapentaenoic Acid/*administration & dosage ; Endothelium, Vascular/*drug effects ; Femoral Artery/*drug effects ; Femoral Vein/*drug effects ; Prostaglandin-Endoperoxide Synthases/*chemistry ; Vascular Diseases/*drug therapy; Administration, Oral ; Animals ; Cyclooxygenase Inhibitors/pharmacology ; Endothelium, Vascular/metabolism ; Endothelium, Vascular/pathology ; Femoral Artery/metabolism ; Femoral Artery/pathology ; Femoral Vein/metabolism ; Femoral Vein/pathology ; Male ; Rats ; Rats, Wistar ; Vascular Diseases/metabolism ; Vascular Diseases/pathology
    • Abstract:
      In humans, aging is associated with endothelial dysfunction and an increased risk of venous thromboembolism. Although intake of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) at a ratio of 6:1 by old rats improved the endothelial dysfunction in arteries, the impact on veins remains unclear. Eight-month-old male Wistar rats were either untreated or orally administered corn oil, EPA:DHA 1:1, or EPA:DHA 6:1 (500 mg/kg/d) for seven days. Vascular reactivity was studied by myography. In middle-aged femoral artery rings, acetylcholine caused a partial relaxation at low concentrations and a contractile response at high concentrations, whereas in the old femoral vein only a partial relaxation was observed. The EPA:DHA 6:1 treatment blunted the contractile response to acetylcholine in the middle-aged femoral artery and both EPA:DHA 6:1 and 1:1 increased the relaxation to acetylcholine in the old femoral vein. No such effects were observed with corn oil. Both the non-selective cyclooxygenase inhibitor indomethacin and the COX-1 inhibitor SC-560 increased the relaxation to acetylcholine in the middle-aged femoral artery whereas the COX-2 inhibitor NS-398 increased that in the middle-aged femoral vein. In conclusion, our results indicate that aging is associated with an endothelial dysfunction in the femoral artery and vein, which can be improved by EPA:DHA 6:1 treatment-most likely via a cyclooxygenase-dependent mechanism.
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    • Contributed Indexing:
      Keywords: aging; cyclooxygenases; endothelial dysfunction; omega-3 polyunsaturated fatty acids; venous thrombosis
    • Accession Number:
      0 (Cyclooxygenase Inhibitors)
      25167-62-8 (Docosahexaenoic Acids)
      AAN7QOV9EA (Eicosapentaenoic Acid)
      EC 1.14.99.1 (Prostaglandin-Endoperoxide Synthases)
    • Publication Date:
      Date Created: 20200206 Date Completed: 20201117 Latest Revision: 20201117
    • Publication Date:
      20221213
    • Accession Number:
      PMC7037507
    • Accession Number:
      10.3390/ijms21030920
    • Accession Number:
      32019237