Polycalin is involved in the toxicity and resistance to Cry1Ac toxin in Helicoverpa armigera (Hübner).

Publisher: Wiley Country of Publication: United States NLM ID: 8501752 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6327 (Electronic) Linking ISSN: 07394462 NLM ISO Abbreviation: Arch Insect Biochem Physiol Subsets: MEDLINE

Publication: New York, NY : Wiley
Original Publication: New York : Alan R. Liss, c1983-

Insecticides/*pharmacology ; Lipocalins/*metabolism ; Moths/*drug effects ; Moths/*physiology; Animals ; Bacterial Proteins/metabolism ; Bacterial Proteins/pharmacology ; Endotoxins/metabolism ; Endotoxins/pharmacology ; Hemolysin Proteins/metabolism ; Hemolysin Proteins/pharmacology ; Insect Proteins ; Insecticide Resistance/genetics ; Insecticides/metabolism ; Moths/metabolism

Polycalin has been confirmed as a binding protein of the Cry toxins in a few Lepidoptera insects, but its function in the action mechanism of Cry1Ac and whether it is involved in resistance evolution are still unclear. In this study, Ligand blot and enzyme-linked immunosorbent assays showed that Helicoverpa armigera polycalin could specifically interact with Cry1Ac with a high affinity (K d  = 118.80 nM). Importantly, antisera blocking polycalin in H. armigera larvae decreased the toxicity of Cry1Ac by 31.84%. Furthermore, the relative gene and protein expressions were lower in Cry1Ac-resistant strain (LF60) than that in Cry1Ac-susceptible strain (LF). These findings indicated that H. armigera polycalin was a possible receptor of Cry1Ac and may be contributed to the resistance to Cry1Ac.
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2016ZX08011-002 Key Project for Breeding Genetically Modified Organisms; 31621064 National Natural Science Foundation of China

Keywords: Helicoverpa armigera; functional receptor; polycalin; resistance mechanisms

0 (Bacterial Proteins)
0 (Endotoxins)
0 (Hemolysin Proteins)
0 (Insect Proteins)
0 (Insecticides)
0 (Lipocalins)

Date Created: 20200204 Date Completed: 20200710 Latest Revision: 20200710

20231215

10.1002/arch.21661

32011765