Class C1 decoy oligodeoxynucleotide inhibits profibrotic genes expression in rat hepatic stellate cells.

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  • Additional Information
    • Source:
      Publisher: D. A. Spandidos Country of Publication: Greece NLM ID: 101475259 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1791-3004 (Electronic) Linking ISSN: 17912997 NLM ISO Abbreviation: Mol Med Rep Subsets: MEDLINE
    • Publication Information:
      Original Publication: Athens, Greece : D. A. Spandidos
    • Subject Terms:
      Gene Expression Regulation*/drug effects; Hepatic Stellate Cells/*metabolism ; Hepatic Stellate Cells/*pathology ; Liver Cirrhosis/*drug therapy ; Liver Cirrhosis/*genetics ; Oligodeoxyribonucleotides/*therapeutic use; Animals ; Cell Line ; Collagen Type I/metabolism ; Down-Regulation/drug effects ; Down-Regulation/genetics ; Hepatic Stellate Cells/drug effects ; Oligodeoxyribonucleotides/pharmacology ; Promoter Regions, Genetic/genetics ; Rats ; Smad3 Protein/metabolism ; Tissue Inhibitor of Metalloproteinase-1/metabolism ; Transcription Factor HES-1/genetics ; Transcription Factor HES-1/metabolism ; Transforming Growth Factor beta/metabolism
    • Abstract:
      The aim of the present study was to investigate whether class C1 decoy oligodeoxynucleotides (ODNs) can inhibit the expression of pro‑fibrotic genes associated with rat hepatic stellate cell (HSC) activation and hepatic fibrosis. Luciferase reporter assays were performed to test the promoter activities of transforming growth factor (TGF)‑β and its downstream target genes following transfection of decoy ODNs and plasmids into HSC‑T6 cells, and western blot assays were performed to measure the protein expression of those genes following decoy ODN transfection. Class C1 decoy ODNs were confirmed to inhibit the promoter activity of TGF‑β and its downstream target genes, such as type 1 collagen (COLI)α1, tissue inhibitor of metalloproteinases (TIMP)1 and α‑smooth muscle actin by Gaussia luciferase reporter assay, and to further downregulate the expression of TGF‑β, SMAD3, COLIα1 and TIMP1 by western blotting in activated HSC‑T6 cells. In conclusion, class C1 decoy ODNs inhibited pro‑fibrotic gene expression in rat HSCS by downregulating TGF‑β signaling.
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    • Contributed Indexing:
      Keywords: class c1 decoy oligodeoxynucleotides; transforming growth factor-β signaling; hepatic stellate cells
    • Accession Number:
      0 (Collagen Type I)
      0 (Hes1 protein, rat)
      0 (Oligodeoxyribonucleotides)
      0 (Smad3 Protein)
      0 (Tissue Inhibitor of Metalloproteinase-1)
      0 (Transcription Factor HES-1)
      0 (Transforming Growth Factor beta)
    • Publication Date:
      Date Created: 20200125 Date Completed: 20201006 Latest Revision: 20210917
    • Publication Date:
      20240829
    • Accession Number:
      PMC6947877
    • Accession Number:
      10.3892/mmr.2019.10881
    • Accession Number:
      31974596