Item request has been placed!
×
Item request cannot be made.
×
Processing Request
Live vaccine consisting of attenuated Salmonella secreting and delivering Brucella ribosomal protein L7/L12 induces humoral and cellular immune responses and protects mice against virulent Brucella abortus 544 challenge.
Item request has been placed!
×
Item request cannot be made.
×
Processing Request
- Additional Information
- Source:
Publisher: BioMed Central Country of Publication: England NLM ID: 9309551 Publication Model: Electronic Cited Medium: Internet ISSN: 1297-9716 (Electronic) Linking ISSN: 09284249 NLM ISO Abbreviation: Vet Res Subsets: MEDLINE
- Publication Information:
Publication: 2011- : London : BioMed Central
Original Publication: Paris : Editions Scientifiques Elsevier ; INRA, c1993-
- Subject Terms:
- Abstract:
The present study employs the Brucella abortus L7/L12 antigen in a Salmonella secretion platform and investigates its ability to induce protective immune responses against wild type challenge in BALB/c mice. The highly conserved L7/L12 open reading frame was PCR amplified from B. abortus and cloned into a prokaryotic expression vector, pJHL65, directly under the beta-lactamase secretory signal. The plasmid constructs pJHL65::L7/L12 was then transformed into an attenuated Salmonella Typhimurium strain, JOL1800 (∆lon, ∆cpxR, ∆asd, and ∆rfaL), and protein secretion was verified by Western blot. Three mice groups were inoculated with either phosphate-buffered saline (PBS), vector-only control, or the vaccine strain secreting L7/L12 antigen. Assessment of humoral and cell-mediated immune responses revealed successful elicitation of Brucella antigen-specific Th1 and Th2 immune responses that were significantly higher than PBS and vector control groups. The immune responses were confirmed by splenocyte proliferation assay, flow cytometry analysis for CD4+ and CD8+ markers, and RT-PCR based cytokine profiling upon restimulation with L7/L12 purified antigen. Results indicate that immunization with Salmonella secreting L7/L12 antigen demonstrated significant enhancement of cell-mediated immune (CMI) responses in immunized mice. The overall effectiveness of the immunization was evaluated by challenging with virulent B. abortus that revealed significant reduction in Brucella colonization in spleen and liver tissues in Salmonella L7/L12 immunized mice. Delivery of Brucella protective antigen L7/L12 using the Salmonella secretion system can effectively accomplish immunogenic advantages of both Salmonella and L7/L12 to derive robust CMI responses and induce humoral immunity to protect against Brucella infection in the mouse model.
- References:
Expert Rev Vaccines. 2012 Feb;11(2):189-209. (PMID: 22309668)
J Pharmacol Pharmacother. 2010 Jul;1(2):87-93. (PMID: 21350616)
Oncotarget. 2018 May 22;9(39):25441-25457. (PMID: 29876000)
Infect Immun. 2006 Sep;74(9):5035-46. (PMID: 16926395)
J Immunol. 2014 Apr 15;192(8):3740-52. (PMID: 24646742)
Nat Rev Microbiol. 2012 Apr 02;10(5):336-51. (PMID: 22466878)
Appl Environ Microbiol. 2002 Feb;68(2):910-6. (PMID: 11823235)
Future Microbiol. 2015;10(1):101-10. (PMID: 25598340)
Vet Res. 2018 Jul 5;49(1):57. (PMID: 29976253)
Vet Microbiol. 2017 Jun;205:84-91. (PMID: 28622869)
Front Biol (Beijing). 2013 Feb 1;8(1):60-77. (PMID: 23730309)
Cytokine. 2005 Apr 21;30(2):86-92. (PMID: 15804600)
Infect Immun. 1990 Jul;58(7):2320-8. (PMID: 2114362)
PLoS One. 2011 Jan 14;6(1):e16203. (PMID: 21264260)
Immunology. 2001 Aug;103(4):511-8. (PMID: 11529943)
Expert Rev Vaccines. 2013 Sep;12(9):1033-45. (PMID: 24053397)
PLoS Pathog. 2013 Sep;9(9):e1003532. (PMID: 24068916)
Iran J Immunol. 2009 Mar;6(1):12-21. (PMID: 19293473)
Infect Immun. 2011 Oct;79(10):4165-74. (PMID: 21768283)
J Proteome Res. 2009 Jan;8(1):113-7. (PMID: 19072545)
Sci Rep. 2017 Mar 23;7:45139. (PMID: 28332591)
Nucleic Acids Res. 2001 May 1;29(9):e45. (PMID: 11328886)
Clin Vaccine Immunol. 2011 Feb;18(2):203-9. (PMID: 21159921)
Germs. 2013 Mar 01;3(1):26-35. (PMID: 24432284)
Vet Immunol Immunopathol. 2019 Mar;209:31-36. (PMID: 30885303)
J Immunol Methods. 1986 May 22;89(2):271-7. (PMID: 3486233)
Vet Microbiol. 2017 Jun;205:117-123. (PMID: 28622853)
- Grant Information:
2019R1A6A1A03033084 National Research Foundation of Korea
- Accession Number:
0 (Antigens, Bacterial)
0 (Brucella Vaccine)
0 (Ribosomal Proteins)
0 (Vaccines, Attenuated)
70815-33-7 (ribosomal protein L7-L12)
- Publication Date:
Date Created: 20200125 Date Completed: 20200727 Latest Revision: 20200727
- Publication Date:
20231215
- Accession Number:
PMC6979349
- Accession Number:
10.1186/s13567-020-0735-y
- Accession Number:
31973749
No Comments.