Pinpointing the role of Aeromonas salmonicida in the development of skin ulcerations in common dab (Limanda limanda).

Publisher: Blackwell Scientific Publications Country of Publication: England NLM ID: 9881188 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2761 (Electronic) Linking ISSN: 01407775 NLM ISO Abbreviation: J Fish Dis Subsets: MEDLINE

Original Publication: Oxford, Blackwell Scientific Publications.

Flounder*; Aeromonas salmonicida/*physiology ; Fish Diseases/*microbiology ; Gram-Negative Bacterial Infections/*veterinary ; Skin Diseases, Bacterial/*veterinary ; Skin Ulcer/*veterinary; Animals ; Gram-Negative Bacterial Infections/microbiology ; Skin Diseases, Bacterial/microbiology ; Skin Ulcer/microbiology

Aeromonas salmonicida was isolated from ulcerations in common dab (Limanda limanda). An experiment was performed to pinpoint its role in ulceration development, considering the importance of the skin barrier and the pigmented and non-pigmented sides. The skin of dab was treated in three zones, one where scales and epidermis were removed, one where mucus was discarded and one non-treated zone. Fish were tagged to allow individual identification and challenged with A. salmonicida. Mortality and severity of the developing lesions were recorded for 21 days post-inoculation. Starting 12 days post-inoculation, mortality occurred gradually in challenged fish; however, no direct cause could be established. Both control fish and challenged fish developed ulcerations containing A. salmonicida. Sequencing of vapA gene revealed that isolates retrieved from both groups were distinct, suggesting the presence of A. salmonicida prior to the trial. Most ulcerations developed in zones where skin was removed, suggesting that abrasion might be a predisposing factor in ulceration development. Ulcerations were also observed at the insertion site of the tag, where exposed muscle tissue might have favoured the development of ulcerations. In conclusion, A. salmonicida seems to be involved in the development of skin ulcerations in dab, although the exact pathogenesis needs to be elucidated.
(© 2020 John Wiley & Sons Ltd.)

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Vlaamse regering; EMBRC-ERIC; VIS/15/A03/DIV EUROPEAN Fisheries Fund; Research Foundation - Flanders (FWO)

Keywords: Aeromonas salmonicida; A-layer typing; common dab; skin barrier; skin ulceration

GENBANK MK244113; MK244206; NVI-09978; MK243982

Date Created: 20200110 Date Completed: 20200812 Latest Revision: 20200930

20240829

10.1111/jfd.13133

31918449