Steady expression of high oleic acid in peanut bred by marker-assisted backcrossing for fatty acid desaturase mutant alleles and its effect on seed germination along with other seedling traits.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read Online Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
    • Publication Information:
      Original Publication: San Francisco, CA : Public Library of Science
    • Subject Terms:
      Germination* ; Mutation*; Arachis/*metabolism ; Fatty Acid Desaturases/*metabolism ; Oleic Acid/*metabolism ; Seedlings/*metabolism ; Seeds/*metabolism; Alleles ; Arachis/genetics ; Arachis/growth & development ; Biomarkers/analysis ; Fatty Acid Desaturases/genetics ; Peanut Oil/analysis ; Plant Breeding ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Seedlings/genetics ; Seedlings/growth & development ; Seeds/genetics ; Seeds/growth & development
    • Abstract:
      Peanut (Arachis hypogaea L.) is an important nutrient-rich food legume and valued for its good quality cooking oil. The fatty acid content is the major determinant of the quality of the edible oil. The oils containing higher monounsaturated fatty acid are preferred for improved shelf life and potential health benefits. Therefore, a high oleic/linoleic fatty acid ratio is the target trait in an advanced breeding program. The two mutant alleles, ahFAD2A (on linkage group a09) and ahFAD2B (on linkage group b09) control fatty acid composition for higher oleic/linoleic ratio in peanut. In the present study, marker-assisted backcrossing was employed for the introgression of two FAD2 mutant alleles from SunOleic95R into the chromosome of ICGV06100, a high oil content peanut breeding line. In the marker-assisted backcrossing-introgression lines, a 97% increase in oleic acid, and a 92% reduction in linoleic acid content was observed in comparison to the recurrent parent. Besides, the oleic/linoleic ratio was increased to 25 with respect to the recurrent parent, which was only 1.2. The most significant outcome was the stable expression of oil-content, oleic acid, linoleic acid, and palmitic acid in the marker-assisted backcrossing-introgression lines over the locations. No significant difference was observed between high oleic and normal oleic in peanuts for seedling traits except germination percentage. In addition, marker-assisted backcrossing-introgression lines exhibited higher yield and resistance to foliar fungal diseases, i.e., late leaf spot and rust.
      Competing Interests: All authors have contributed equally to this research work and have no competing interests exist.
    • References:
      Mol Gen Genet. 2000 Jun;263(5):796-805. (PMID: 10905347)
      Biotechnol Adv. 2012 May-Jun;30(3):639-51. (PMID: 22094114)
      Plant Physiol. 1976 Jul;58(1):7-11. (PMID: 16659623)
      World Health Organ Tech Rep Ser. 2003;916:i-viii, 1-149, backcover. (PMID: 12768890)
      PLoS One. 2012;7(7):e41213. (PMID: 22815973)
      Plant Physiol. 1973 Dec;52(6):549-53. (PMID: 16658603)
      BMC Genet. 2014 Dec 10;15:133. (PMID: 25491595)
      J Food Sci Technol. 2016 Jan;53(1):31-41. (PMID: 26787930)
      Mol Gen Genet. 2000 Jun;263(5):806-11. (PMID: 10905348)
      Metabolites. 2019 Feb 22;9(2):null. (PMID: 30813306)
      PLoS One. 2015 Apr 07;10(4):e0119454. (PMID: 25849082)
      Plant Sci. 2016 Jan;242:203-213. (PMID: 26566838)
      Plant Cell Physiol. 1993 Jun;34(4):605-12. (PMID: 8025825)
      Biotechnol Biotechnol Equip. 2015 Mar 4;29(2):237-254. (PMID: 26019637)
      Biotechnol Adv. 2013 Dec;31(8):1120-34. (PMID: 23313999)
      Lipids. 1997 Jul;32(7):687-95. (PMID: 9252956)
      Eur J Clin Nutr. 2002 Jan;56(1):72-81. (PMID: 11840183)
      J Agric Food Chem. 2002 Feb 27;50(5):1298-305. (PMID: 11853521)
      Theor Appl Genet. 2014 Aug;127(8):1771-81. (PMID: 24927821)
      J Agric Food Chem. 2013 Mar 20;61(11):2875-82. (PMID: 23379758)
      Lipids Health Dis. 2009 Jun 26;8:25. (PMID: 19558671)
      Front Plant Sci. 2016 Aug 25;7:1271. (PMID: 27610115)
    • Accession Number:
      0 (Biomarkers)
      0 (Peanut Oil)
      0 (Plant Proteins)
      2UMI9U37CP (Oleic Acid)
      EC 1.14.19.- (Fatty Acid Desaturases)
    • Publication Date:
      Date Created: 20191213 Date Completed: 20200326 Latest Revision: 20200326
    • Publication Date:
      20221213
    • Accession Number:
      PMC6910123
    • Accession Number:
      10.1371/journal.pone.0226252
    • Accession Number:
      31830093