Structure characterization of high molecular weight soluble dietary fiber from mushroom Lentinula edodes (Berk.) Pegler and its interaction mechanism with pancreatic lipase and bile salts.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE
    • Publication Information:
      Publication: Amsterdam : Elsevier
      Original Publication: Guildford, Eng., IPC Science and Technology Press.
    • Subject Terms:
      Bile Acids and Salts/*metabolism ; Dietary Fiber/*metabolism ; Lipase/*metabolism ; Shiitake Mushrooms/*chemistry; Dietary Fiber/pharmacology ; Digestion ; Hep G2 Cells ; Humans ; Molecular Weight ; Solubility
    • Abstract:
      Aimed to evaluate the hypolipidemic effects of high molecular weight soluble dietary fiber extracted from L. edodes (LEHSDF), this study investigated the structure and interaction mechanism of LEHSDF with pancreatic lipase (PL) and bile salts (BS) that were involved in lipid digestion. 1D/2D NMR spectra indicated that the main chain of LEHSDF consisted of (1 → 2,4)-linked β-D-arabinopyranosyl, (1 → 3)-linked α-L-rhamnopyranosyl, (1 → 4)-linked β-D-xylopyranosyl, (1 → 6)-linked and (1 → 4)-linked β-D-glucopyranosyl, with β-D-galactopyranosyl and α-D-mannopyranosyl as terminal unit. Oil red O staining results suggested that LEHSDF had an effective inhibitory effect on lipid accumulation in HepG2 cells. Isothermal titration calorimetry, fluorescence and circular dichroism spectra showed that BS did not specifically bind to LEHSDF, and the strong inhibitory effect of LEHSDF on lipase was dominated by hydrophobic forces, electrostatic forces, encapsulation and adsorption interactions. The results will be helpful for the design of food containing LEHSDF as a functional additive to control lipid digestion.
      Competing Interests: Declaration of Competing Interest None.
      (Copyright © 2019 Elsevier B.V. All rights reserved.)
    • Contributed Indexing:
      Keywords: Hypolipidemic; ITC; Interaction mechanism; Lentinula edodes; Pancreatic lipase; Soluble dietary fiber
    • Accession Number:
      0 (Bile Acids and Salts)
      0 (Dietary Fiber)
      EC 3.1.1.3 (Lipase)
    • Publication Date:
      Date Created: 20191124 Date Completed: 20210209 Latest Revision: 20210209
    • Publication Date:
      20231215
    • Accession Number:
      10.1016/j.ijbiomac.2019.10.263
    • Accession Number:
      31758996