Molecular Detection of Feline Leukemia Virus in Oral, Conjunctival, and Rectal Mucosae Provides Results Comparable to Detection in Blood.

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  • Additional Information
    • Source:
      Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 7505564 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 1098-660X (Electronic) Linking ISSN: 00951137 NLM ISO Abbreviation: J Clin Microbiol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Washington, American Society for Microbiology.
    • Subject Terms:
      Leukemia Virus, Feline/*isolation & purification ; Molecular Diagnostic Techniques/*veterinary ; Mucous Membrane/*virology ; Polymerase Chain Reaction/*veterinary ; Retroviridae Infections/*veterinary ; Tumor Virus Infections/*veterinary; Animals ; Cat Diseases/diagnosis ; Cat Diseases/virology ; Cats ; Conjunctiva/virology ; DNA, Viral/genetics ; Leukemia Virus, Feline/genetics ; Mouth/virology ; Proviruses/genetics ; Rectum/virology ; Retroviridae Infections/diagnosis ; Tumor Virus Infections/diagnosis ; Viral Load
    • Abstract:
      Feline leukemia virus (FeLV) infection causes immunosuppression, degeneration of the hematopoietic system, and fatal neoplasms. FeLV transmission occurs mainly by close social contact of infected and susceptible cats. Developing procedures for the diagnosis of feline retroviruses is crucial to reduce negative impacts on cat health and increase the number of animals tested. Blood collection requires physical or chemical restraint and is usually a stressful procedure for cats. Our objective was to evaluate the use of samples obtained from oral, conjunctival, and rectal mucosae for the molecular diagnosis of FeLV. Whole blood and oral, conjunctival, and rectal swabs were collected from a total of 145 cats. All samples were subjected to the amplification of a fragment of the gag gene of proviral DNA. Compared to blood samples used in this study as a reference, the accuracies for each PCR were 91.72, 91.23, and 85.50% for samples obtained by oral, conjunctival, and rectal swabs, respectively. The diagnostic sensitivity and specificity were 86.11 and 97.26% for the oral swabs, 90 and 92.59% for the conjunctival swabs, and 74.24 and 95.77% for the rectal swabs, respectively. The kappa values for oral, conjunctival, and rectal swabs were 0.834, 0.824, and 0.705, respectively. The diagnosis of these samples showed the presence of proviral DNA of FeLV in oral and conjunctival mucosae. In conclusion, mucosal samples for the molecular diagnosis of FeLV are an excellent alternative to venipuncture and can be safely used. It is faster, less laborious, less expensive, and well received by the animal.
      (Copyright © 2020 American Society for Microbiology.)
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    • Contributed Indexing:
      Keywords: FeLV; PCR; conjunctival swab; oral swab; rectal swab
    • Accession Number:
      0 (DNA, Viral)
    • Publication Date:
      Date Created: 20191122 Date Completed: 20210406 Latest Revision: 20210406
    • Publication Date:
      20240829
    • Accession Number:
      PMC6989071
    • Accession Number:
      10.1128/JCM.01233-19
    • Accession Number:
      31748326