Bacitracin resistance and enhanced virulence of Streptococcus suis via a novel efflux pump.

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  • Additional Information
    • Source:
      Publisher: BioMed Central Country of Publication: England NLM ID: 101249759 Publication Model: Electronic Cited Medium: Internet ISSN: 1746-6148 (Electronic) Linking ISSN: 17466148 NLM ISO Abbreviation: BMC Vet Res Subsets: MEDLINE
    • Publication Information:
      Original Publication: London : BioMed Central, 2005-
    • Subject Terms:
      Drug Resistance, Bacterial*; Anti-Bacterial Agents/*pharmacology ; Bacitracin/*pharmacology ; Bacterial Proteins/*metabolism ; Membrane Transport Proteins/*metabolism ; Streptococcus suis/*drug effects; Animals ; Anti-Bacterial Agents/metabolism ; Anti-Infective Agents, Local/metabolism ; Anti-Infective Agents, Local/pharmacology ; Bacitracin/metabolism ; Bacterial Proteins/genetics ; Female ; Gene Deletion ; Gene Expression Regulation, Bacterial ; Membrane Transport Proteins/genetics ; Mice ; Mice, Inbred BALB C ; Microbial Sensitivity Tests ; Streptococcal Infections/microbiology ; Streptococcus suis/metabolism ; Streptococcus suis/pathogenicity ; Virulence
    • Abstract:
      Background: Streptococcus suis is a prominent pathogen causing septicemia and meningitis in swine and humans. Bacitracin is used widely as a growth promoter in animal feed and to control the spread of necrotic enteritis in most developing countries. This study aimed to characterize a novel membrane transporter module Sst comprising SstE, SstF, and SstG for bacitracin resistance.
      Results: Comparative genomics and protein homology analysis found a potential efflux pump SstFEG encoded upstream of well-known bacitracin-resistance genes bceAB and bceRS. A four-fold decrease in bacitracin susceptibility was observed in sstFEG deletion mutant comparing with S. suis wildtype strain CZ130302. Further studies indicated that the bacitracin tolerance mediated by SstFEG is not only independent of the BceAB transporter, but also regulated by the two-component system BceSR. Given that SstFEG are harbored by almost all virulent strains, but not in the avirulent strains, we managed to explore its potential role in bacterial pathogencity. Indeed, our results showed that SstFEG is involved in S. suis colonization and virulence in animal infection model by its potential competitive survival advantage against host bactericidal effect.
      Conclusion: To our knowledge, this is the first study to functionally characterize the bacitracin efflux pump in S. suis to provide evidence regarding the important roles of the novel ABC transporter system SstFEG with respect to drug resistance and virulence.
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    • Grant Information:
      BK20191309 Natural Science Foundation of Jiangsu Province; G2016060201 Shanghai Agriculture Applied Technology Development Program; 0 Priority Academic Program Development of Jiangsu Higher Education Institutions
    • Contributed Indexing:
      Keywords: Bacitracin; Efflux pump; Serotype Chz; SstFEG; Streptococcus suis; Virulence
    • Accession Number:
      0 (Anti-Bacterial Agents)
      0 (Anti-Infective Agents, Local)
      0 (Bacterial Proteins)
      0 (Membrane Transport Proteins)
      1405-87-4 (Bacitracin)
    • Publication Date:
      Date Created: 20191030 Date Completed: 20200313 Latest Revision: 20200313
    • Publication Date:
      20221213
    • Accession Number:
      PMC6819616
    • Accession Number:
      10.1186/s12917-019-2115-2
    • Accession Number:
      31660968