A statistical model for activation of Factor C by binding to LPS aggregates.

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  • Additional Information
    • Source:
      Publisher: Springer Verlag and the European Biophysical Societies Association Country of Publication: Germany NLM ID: 8409413 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-1017 (Electronic) Linking ISSN: 01757571 NLM ISO Abbreviation: Eur Biophys J Subsets: MEDLINE
    • Publication Information:
      Publication: 1996- : Berlin : Springer Verlag and the European Biophysical Societies Association
      Original Publication: Berlin ; New York : Springer International, c1984-
    • Subject Terms:
      Models, Molecular*; Arthropod Proteins/*metabolism ; Enzyme Precursors/*metabolism ; Lipopolysaccharides/*metabolism ; Lipopolysaccharides/*pharmacology ; Serine Endopeptidases/*metabolism; Arthropod Proteins/chemistry ; Enzyme Precursors/chemistry ; Lipid A/metabolism ; Lipid A/pharmacology ; Lipopolysaccharides/chemistry ; Protein Binding ; Protein Multimerization/drug effects ; Protein Structure, Quaternary ; Serine Endopeptidases/chemistry
    • Abstract:
      Published data on Factor C activity at various LPS and Lipid A concentrations (Nakamura et al. in Eur J Biochem 176:89, 1988; Kobayashi et al. in J Biol Chem 37:25987, 2014) were rearranged to show that Factor C exhibited its maximum activity at a specific concentration of LPS. A statistical model was proposed for examining whether a single LPS molecule binding activates Factor C (monomeric activation) or dimerization of Factor C is necessary for the activation (dimeric activation). In the monomeric activation model the plots of the relative activity of Factor C against the molar ratio of LPS to Factor C were different from those in the published data. The plots in the dimeric activation model lie on a bell-shaped curve, whatever the Factor C concentration, matching the published data and indicating the appropriateness of that model. We suggest that Factor C is activated by multiple molecular interactions of Factor C with LPS aggregates on which it dimerises and that this explains why larger aggregates are less effective at activating Factor C than smaller ones.
    • References:
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    • Grant Information:
      JP18J01966 Japan Society for the Promotion of Science
    • Contributed Indexing:
      Keywords: Endotoxin; Factor C; Limulus amebocyte lysate; Lipopolysaccharide; Statistical model
    • Accession Number:
      0 (Arthropod Proteins)
      0 (Enzyme Precursors)
      0 (Lipid A)
      0 (Lipopolysaccharides)
      EC 3.4.21.- (Serine Endopeptidases)
      EC 3.4.21.84 (Limulus clotting factor C)
    • Publication Date:
      Date Created: 20191021 Date Completed: 20200323 Latest Revision: 20200323
    • Publication Date:
      20240829
    • Accession Number:
      10.1007/s00249-019-01400-4
    • Accession Number:
      31630219