Thromboelastometric evaluation of coagulation profiles of cold-stored autologous whole blood: A prospective observational study.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Lippincott Williams & Wilkins Country of Publication: United States NLM ID: 2985248R Publication Model: Print Cited Medium: Internet ISSN: 1536-5964 (Electronic) Linking ISSN: 00257974 NLM ISO Abbreviation: Medicine (Baltimore) Subsets: MEDLINE
    • Publication Information:
      Original Publication: Hagerstown, Md : Lippincott Williams & Wilkins
    • Subject Terms:
      Blood Coagulation* ; Whole Blood Coagulation Time*; Blood Transfusion/*methods ; Thrombelastography/*methods; Aged ; Blood Coagulation Tests ; Cold Temperature ; Female ; Fibrin/chemistry ; Humans ; Male ; Middle Aged ; Platelet Count ; Polymerization ; Prospective Studies
    • Abstract:
      Preoperative autologous blood donation is a well-established procedure to reduce the need for allogeneic blood transfusion. We hypothesized that coagulation activity is maintained in cold-stored whole blood, because the fundamental polymerization properties of fibrin are preserved.Fifty adult patients who underwent elective cardiothoracic surgery were enrolled.Autologous whole blood collected 2 to 4 times at almost 1-week intervals before surgery was stored at approximately 4°C until reinfusion at the time of surgery. Blood samples were drawn just before reinfusion, and rotational thromboelastometry variables and fibrinogen levels were measured.A total of 158 samples were analyzed. The mean duration of cold storage was 16.7 ± 7.4 days (range: 6-33 days). Platelet counts were very low due to collection through a leukoreduction filter. The mean fibrinogen level was 2.3 ± 0.6 g/L. Amplitude at 10 minutes after CT (A10), amplitude at 20 minutes after CT (A20), and maximum clot firmness (MCF) values as determined by FIBTEM analysis were 10.8 ± 3.8, 12.2 ± 4.2, and 13.1 ± 4.7 mm, respectively. Fibrinogen levels were strongly correlated with A10, A20, and FIBTEM-MCF values (ρ = 0.83, P < .0001, ρ = 0.84, P < .0001, ρ = 0.85, P < .0001, respectively). Fibrinogen levels were not correlated with the duration of cold storage (ρ = 0.06, P = .43).The results of the present study demonstrate that fibrin polymerization occurs in cold-stored autologous whole blood, and that such activity is strongly correlated with fibrinogen levels. Furthermore, our data suggest that cold-stored leukoreduced autologous whole blood retains fibrin polymerization properties throughout 33 days.
    • References:
      J Cardiothorac Vasc Anesth. 2013 Aug;27(4 Suppl):S20-34. (PMID: 23910533)
      Transfusion. 2013 Jan;53 Suppl 1:137S-149S. (PMID: 23301966)
      J Cardiothorac Vasc Anesth. 2012 Dec;26(6):1083-93. (PMID: 22863406)
      J Cardiothorac Vasc Anesth. 2005 Oct;19(5):589-96. (PMID: 16202891)
      Transfus Med Rev. 2012 Jan;26(1):1-13. (PMID: 21872428)
      Blood Coagul Fibrinolysis. 2005 Jun;16(4):301-10. (PMID: 15870552)
      Transfusion. 2010 Jan;50(1):185-9. (PMID: 19694993)
      Arch Pathol Lab Med. 2003 Mar;127(3):316-20. (PMID: 12653575)
      J Cardiothorac Vasc Anesth. 2014 Apr;28(2):210-6. (PMID: 24630470)
      N Engl J Med. 1995 Mar 16;332(11):719-24. (PMID: 7854380)
      Transfusion. 2012 Jan;52(1):14-22. (PMID: 21756263)
      Transfusion. 1998 Mar;38(3):296-300. (PMID: 9563411)
      Anesthesiology. 2010 Nov;113(5):1205-19. (PMID: 20881594)
      Br J Anaesth. 2010 Oct;105(4):429-36. (PMID: 20693180)
      Br J Haematol. 2013 Nov;163(3):303-14. (PMID: 24032719)
      Thromb Res. 2015 Sep;136(3):669-72. (PMID: 26232350)
      Transfusion. 2011 Jan;51(1):43-51. (PMID: 20663116)
      J Cardiothorac Vasc Anesth. 2013 Aug;27(4 Suppl):S9-15. (PMID: 23910538)
      Anesth Analg. 2013 Aug;117(2):314-21. (PMID: 23780419)
      Transfusion. 2008 Oct;48(10):2133-42. (PMID: 18564391)
      Br J Anaesth. 2016 May;116(5):618-23. (PMID: 26893405)
      Br J Anaesth. 2009 Dec;103 Suppl 1:i14-22. (PMID: 20007984)
      Ann Thorac Surg. 2011 Mar;91(3):944-82. (PMID: 21353044)
      Transfusion. 2006 May;46(5):811-7. (PMID: 16686849)
      Transfus Med. 2018 Apr;28(2):168-180. (PMID: 28940923)
      Transfus Apher Sci. 2013 Dec;49(3):673-80. (PMID: 23491867)
      Health Technol Assess. 2006 Nov;10(44):iii-iv, ix-x, 1-210. (PMID: 17049141)
      J Trauma Acute Care Surg. 2015 Jun;78(6 Suppl 1):S31-8. (PMID: 26002261)
      Anesthesiology. 2011 Dec;115(6):1179-91. (PMID: 21970887)
    • Accession Number:
      9001-31-4 (Fibrin)
    • Publication Date:
      Date Created: 20191003 Date Completed: 20191009 Latest Revision: 20221005
    • Publication Date:
      20240829
    • Accession Number:
      PMC6775353
    • Accession Number:
      10.1097/MD.0000000000017357
    • Accession Number:
      31574880