A Convenient Fluorescence-Based Assay for the Detection of Sucrose Transport and the Introduction of a Sucrose Transporter from Potato into Clostridium Strains.

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  • Additional Information
    • Source:
      Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE
    • Publication Information:
      Original Publication: Basel, Switzerland : MDPI, c1995-
    • Subject Terms:
      Biological Assay* ; Gene Expression*; Clostridium/*genetics ; Clostridium/*metabolism ; Membrane Transport Proteins/*genetics ; Plant Proteins/*genetics ; Solanum tuberosum/*metabolism ; Sucrose/*metabolism; Biological Transport ; Fluorescence ; Membrane Transport Proteins/metabolism ; Plant Proteins/metabolism
    • Abstract:
      A convenient and effective sucrose transport assay for Clostridium strains is needed. Traditional methods, such as 14 C-sucrose isotope labelling, use radioactive materials and are not convenient for many laboratories. Here, a sucrose transporter from potato was introduced into Clostridium , and a fluorescence assay based on esculin was used for the analysis of sucrose transport in Clostridium strains. This showed that the heterologously expressed potato sucrose transporter is functional in Clostridium . Recombinant engineering of high-level sucrose transport would aid sucrose fermentation in Clostridium strains. The assay described herein provides an important technological platform for studying sucrose transporter function following heterologous expression in Clostridium .
    • References:
      Biotechnology (N Y). 1992 Feb;10(2):190-5. (PMID: 1368230)
      Plant Cell. 1993 Nov;5(11):1591-8. (PMID: 8312741)
      Bioresour Technol. 2017 Dec;245(Pt B):1469-1475. (PMID: 28583406)
      Appl Environ Microbiol. 2011 May;77(10):3311-9. (PMID: 21421781)
      Appl Microbiol Biotechnol. 2016 Oct;100(19):8255-71. (PMID: 27531513)
      Metab Eng. 2018 Jan;45:121-133. (PMID: 29196124)
      EMBO J. 1992 Dec;11(13):4705-13. (PMID: 1464305)
      Plant J. 1993 Oct;4(4):601-10. (PMID: 8252064)
      Appl Environ Microbiol. 2005 May;71(5):2777-81. (PMID: 15870373)
      J Appl Microbiol. 1998 May;84(5):914-9. (PMID: 9674147)
      Curr Opin Chem Biol. 2008 Apr;12(2):187-93. (PMID: 18275861)
      Plant Physiol. 1989 Apr;89(4):1318-23. (PMID: 16666703)
      Microb Cell Fact. 2009 Dec 15;8:66. (PMID: 20003468)
      Front Plant Sci. 2018 Apr 23;9:430. (PMID: 29740457)
      Plant J. 2011 Oct;68(1):129-36. (PMID: 21668536)
      J Bacteriol. 2003 Mar;185(6):1923-34. (PMID: 12618456)
      BMC Syst Biol. 2011 Jan 19;5:10. (PMID: 21247470)
      Appl Environ Microbiol. 1993 Apr;59(4):1077-81. (PMID: 8386500)
      Bioresour Technol. 2018 Dec;270:430-438. (PMID: 30245312)
      Front Plant Sci. 2012 Feb 15;3:22. (PMID: 22639641)
      Front Microbiol. 2015 Oct 21;6:1165. (PMID: 26539187)
      J Mol Microbiol Biotechnol. 2000 Jan;2(1):71-80. (PMID: 10937490)
      Appl Microbiol Biotechnol. 2016 Jul;100(14):6119-30. (PMID: 27209041)
      Microbiology. 1999 Jun;145 ( Pt 6):1461-72. (PMID: 10411273)
      J Biol Chem. 1996 Oct 11;271(41):25139-44. (PMID: 8810269)
      Appl Environ Microbiol. 2018 Aug 17;84(17):. (PMID: 29959252)
      Appl Microbiol Biotechnol. 2012 Feb;93(4):1485-94. (PMID: 22052388)
      Appl Environ Microbiol. 1999 Feb;65(2):499-505. (PMID: 9925574)
      Plant Methods. 2012 Apr 04;8:13. (PMID: 22475854)
      Appl Environ Microbiol. 2017 Aug 31;83(18):. (PMID: 28687653)
      Plant Physiol. 1974 Dec;54(6):886-91. (PMID: 16658994)
      Metab Eng. 2013 Jul;18:1-8. (PMID: 23541907)
      Appl Environ Microbiol. 2007 Mar;73(6):1842-50. (PMID: 17209069)
      Biotechnol Rep (Amst). 2015 Aug 05;8:1-9. (PMID: 28352567)
      J Biol Chem. 1982 Dec 10;257(23):14538-42. (PMID: 6754735)
    • Grant Information:
      21566003 National Natural Science Foundation of China; 21366007 National Natural Science Foundation of China
    • Contributed Indexing:
      Keywords: Clostridium; esculin; fluorescence assay; sucrose transporter
    • Accession Number:
      0 (Membrane Transport Proteins)
      0 (Plant Proteins)
      0 (sucrose transport protein, plant)
      57-50-1 (Sucrose)
    • Publication Date:
      Date Created: 20190929 Date Completed: 20200212 Latest Revision: 20200212
    • Publication Date:
      20231215
    • Accession Number:
      PMC6803915
    • Accession Number:
      10.3390/molecules24193495
    • Accession Number:
      31561523