Induction of ornithine decarboxylase in guinea-pig lymphocytes. Synergistic effect of diacylglycerol and calcium.

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  • Author(s): Otani S; Matsui I; Kuramoto A; Morisawa S
  • Source:
    European journal of biochemistry [Eur J Biochem] 1985 Feb 15; Vol. 147 (1), pp. 27-31.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies Country of Publication: England NLM ID: 0107600 Publication Model: Print Cited Medium: Print ISSN: 0014-2956 (Print) Linking ISSN: 00142956 NLM ISO Abbreviation: Eur J Biochem Subsets: MEDLINE
    • Publication Information:
      Publication: -2004: Oxford, UK : Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
      Original Publication: Berlin, New York, Springer.
    • Subject Terms:
      Calcium/*physiology ; Diglycerides/*physiology ; Glycerides/*physiology ; Lymphocytes/*enzymology ; Ornithine Decarboxylase/*biosynthesis; Animals ; Calcimycin/pharmacology ; Calcium/metabolism ; Calmodulin/antagonists & inhibitors ; Cells, Cultured ; Cytosol/metabolism ; Diglycerides/metabolism ; Enzyme Induction/drug effects ; Guinea Pigs ; Phorbol Esters/pharmacology ; Phorbols/pharmacology ; Phytohemagglutinins/pharmacology ; Protein Kinase C ; Protein Kinases/metabolism ; Type C Phospholipases/metabolism
    • Abstract:
      Calmodulin antagonists, N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), N-(6-aminohexyl)-1-naphthalenesulfonamide (W-5) and trifluoperazine inhibited ornithine decarboxylase induction in lymphocytes activated with phytohemagglutinin or inophore A23187. W-7, a more potent calmodulin antagonist than W-5, suppressed ornithine decarboxylase induction in a higher extent than did W-5. These results suggest that calmodulin may play an important role in ornithine decarboxylase induction in the activated lymphocytes. However, the extent of ornithine decarboxylase induction was greater in cells pretreated with Clostridium phospholipase C and then incubated with ionophore A23187 than in cells incubated with ionophore A23187 without the pretreatment. Moreover, combined treatment of cells with ionophore A23187 and tumor promotor, phorbol 12-myristate 13-acetate, caused synergistic induction of ornithine decarboxylase activity. These results, taken together, suggest that both activations of Ca2+-activated phospholipid-dependent protein kinase by diacylglycerol and of calmodulin-dependent function resulted from an elevation of cytosolic Ca2+ concentration may operate in the induction of ornithine decarboxylase in the activated lymphocytes.
    • Accession Number:
      0 (Calmodulin)
      0 (Diglycerides)
      0 (Glycerides)
      0 (Phorbol Esters)
      0 (Phorbols)
      0 (Phytohemagglutinins)
      37H9VM9WZL (Calcimycin)
      EC 2.7.- (Protein Kinases)
      EC 2.7.11.13 (Protein Kinase C)
      EC 3.1.4.- (Type C Phospholipases)
      EC 4.1.1.17 (Ornithine Decarboxylase)
      SY7Q814VUP (Calcium)
      XUZ76S9127 (phorbol)
    • Publication Date:
      Date Created: 19850215 Date Completed: 19850410 Latest Revision: 20190620
    • Publication Date:
      20240829
    • Accession Number:
      10.1111/j.1432-1033.1985.tb08713.x
    • Accession Number:
      3156035