Identification and Allelopathy of Green Garlic ( Allium sativum L.) Volatiles on Scavenging of Cucumber ( Cucumis sativus L.) Reactive Oxygen Species.

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  • Additional Information
    • Source:
      Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE
    • Publication Information:
      Original Publication: Basel, Switzerland : MDPI, c1995-
    • Subject Terms:
      Allyl Compounds/*pharmacology ; Cucumis sativus/*metabolism ; Disulfides/*pharmacology ; Garlic/*chemistry ; Reactive Oxygen Species/*analysis; Allyl Compounds/isolation & purification ; Cucumis sativus/drug effects ; Cucumis sativus/genetics ; Disulfides/isolation & purification ; Gene Expression Regulation, Plant/drug effects ; Hydrogen Peroxide/analysis ; Oxidative Stress/drug effects ; Pheromones/isolation & purification ; Pheromones/pharmacology ; Plant Proteins/genetics ; Seedlings/drug effects ; Seedlings/metabolism ; Superoxides/analysis ; Volatile Organic Compounds/chemistry ; Volatile Organic Compounds/isolation & purification
    • Abstract:
      Garlic and formulations containing allicin are used widely as fungicides in modern agriculture. However, limited reports are available on the allelopathic mechanism of green garlic volatile organic compounds (VOCs) and its component allelochemicals. The aim of this study was to investigate VOCs of green garlic and their effect on scavenging of reactive oxygen species (ROS) in cucumber. In this study, green garlic VOCs were collected by HS-SPME, then analyzed by GS-MS. Their biological activity were verified by bioassays. The results showed that diallyl disulfide (DADS) is the main allelochemical of green garlic VOCs and the DADS content released from green garlic is approximately 0.08 mg/g. On this basis, the allelopathic effects of green garlic VOCs in vivo and 1 mmol/L DADS on scavenging of ROS in cucumber seedlings were further studied. Green garlic VOCs and DADS both reduce superoxide anion and increase the accumulation of hydrogen peroxide of cucumber seedlings. They can also regulate active antioxidant enzymes (SOD, CAT, POD), antioxidant substances (MDA, GSH and ASA) and genes ( CscAPX , CsGPX , CsMDAR , CsSOD , CsCAT , CsPOD ) responding to oxidative stress in cucumber seedlings.
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    • Grant Information:
      No. 31772293 Innovative Research Group Project of the National Natural Science Foundation of China
    • Contributed Indexing:
      Keywords: GC-MS; allelochemicals; biological activity; green garlic volatile organic compounds; reactive oxygen species; volatiles isolation and analysis
    • Accession Number:
      0 (Allyl Compounds)
      0 (Disulfides)
      0 (Pheromones)
      0 (Plant Proteins)
      0 (Reactive Oxygen Species)
      0 (Volatile Organic Compounds)
      11062-77-4 (Superoxides)
      5HI47O6OA7 (diallyl disulfide)
      BBX060AN9V (Hydrogen Peroxide)
    • Publication Date:
      Date Created: 20190911 Date Completed: 20200127 Latest Revision: 20200127
    • Publication Date:
      20250114
    • Accession Number:
      PMC6767350
    • Accession Number:
      10.3390/molecules24183263
    • Accession Number:
      31500271