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Phenotypic landscape of granulocytes and monocytes by multiparametric flow cytometry: A prospective study of a 1-tube panel strategy for diagnosis and prognosis of patients with MDS.
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- Author(s): Barreau S;Barreau S;Barreau S; Green AS; Green AS; Dussiau C; Dussiau C; Dussiau C; Alary AS; Alary AS; Alary AS; Raimbault A; Raimbault A; Raimbault A; Mathis S; Mathis S; Mathis S; Willems L; Willems L; Bouscary D; Bouscary D; Bouscary D; Kosmider O; Kosmider O; Kosmider O; Bardet V; Bardet V; Bardet V; Fontenay M; Fontenay M; Fontenay M; Chapuis N; Chapuis N; Chapuis N
- Source:
Cytometry. Part B, Clinical cytometry [Cytometry B Clin Cytom] 2020 May; Vol. 98 (3), pp. 226-237. Date of Electronic Publication: 2019 Sep 09.- Publication Type:
Journal Article- Language:
English - Source:
- Additional Information
- Source: Publisher: Wiley-Liss Country of Publication: United States NLM ID: 101235690 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1552-4957 (Electronic) Linking ISSN: 15524949 NLM ISO Abbreviation: Cytometry B Clin Cytom Subsets: MEDLINE
- Publication Information: Original Publication: Hoboken, N.J. : Wiley-Liss, c2002-
- Subject Terms: Prognosis*; Flow Cytometry/*methods ; Myelodysplastic Syndromes/*diagnosis; Adult ; Aged ; Aged, 80 and over ; Female ; Granulocytes/pathology ; Granulocytes/ultrastructure ; Humans ; Leukocyte Count ; Male ; Middle Aged ; Monocytes/pathology ; Monocytes/ultrastructure ; Myelodysplastic Syndromes/diagnostic imaging ; Myelodysplastic Syndromes/pathology ; Prospective Studies
- Abstract: Background: Multiparametric flow cytometry (MFC) was recently reported to be a helpful additional tool in the diagnosis of myelodysplastic syndromes (MDS). However, numerous aberrancies have been reported that makes their evaluation difficult as part of a routine diagnosis.
Methods: Here, we validated a 1-tube panel for the evaluation of granulocytic and monocytic maturation by MFC and correlated our findings with diagnosis and prognosis of MDS. A total of 251 samples with MDS suspicion were prospectively analyzed and compared to an internal reference database leading to the calculation of the Diff score.
Results: The associated specificity and sensitivity values of this scoring system were 92.1% and 60.4% in a first learning cohort and 96.7% and 65.2% in a second independent validation cohort. The combination of the Diff score with the concomitantly calculated Ogata score increased the sensitivity to 74.2% and 78.3% in the learning and validation cohorts, respectively. Finally, a normal Diff score in MDS patients was associated with a significant prolonged progression-free survival.
Conclusions: Taken together, the present data indicate that our strategy is a sensitive and specific MFC tool for the diagnosis of MDS-related cytopenia(s) which could be also useful for predicting evolution of these diseases.
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Westers, T. M., Ireland, R., Kern, W., Alhan, C., Balleisen, J. S., Bettelheim, P., … van de Loosdrecht, A. A. (2012). Standardization of flow cytometry in myelodysplastic syndromes: A report from an international consortium and the European LeukemiaNet Working Group. Leukemia, 26, 1730-1741. - Contributed Indexing: Keywords: MDS; diagnosis; disease progression; maturation pathways; multiparametric flow cytometry
- Publication Date: Date Created: 20190910 Date Completed: 20210609 Latest Revision: 20210609
- Publication Date: 20221213
- Accession Number: 10.1002/cyto.b.21843
- Accession Number: 31498561
- Source:
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