A point mutation to the long terminal repeat of bovine leukemia virus related to viral productivity and transmissibility.

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  • Additional Information
    • Source:
      Publisher: Academic Press Country of Publication: United States NLM ID: 0110674 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0341 (Electronic) Linking ISSN: 00426822 NLM ISO Abbreviation: Virology Subsets: MEDLINE
    • Publication Information:
      Original Publication: New York, Academic Press.
    • Subject Terms:
      Disease Transmission, Infectious* ; Point Mutation* ; Terminal Repeat Sequences*; Enzootic Bovine Leukosis/*transmission ; Enzootic Bovine Leukosis/*virology ; Leukemia Virus, Bovine/*genetics ; Leukemia Virus, Bovine/*growth & development; Animals ; Cattle ; Cell Line ; Genotype ; Leukemia Virus, Bovine/classification ; Leukemia Virus, Bovine/isolation & purification ; Models, Biological ; Phylogeny ; Reverse Genetics ; Virus Replication ; Whole Genome Sequencing
    • Abstract:
      It is important to establish the molecular basis of the high transmissibility of bovine leukemia virus (BLV) to develop new methods of preventing viral transmission. Hence, the aim of this study was to determine whether some strains had transmission advantages. First, we determined the whole BLV genome sequences of all 34 BLV-infected cows from one farm. Phylogenetic analysis divided strains into 26 major and 8 minor strains. The major strains dominantly spread independent of host factor, bovine leucocyte antigen. Further analysis, with molecular clones, associated transmissibility with viral productivity in vitro. In addition, the two groups could be classified by group-specific mutations. The reverse genetic approach demonstrated that a spontaneous mutation at nucleotide 175 of the BLV genome, which is located in the viral promoter region, could alter viral productivity by changing viral transactivation, suggesting that BLV transmissibility is affected by a spontaneous mutation associated with viral productivity.
      (Copyright © 2019 Elsevier Inc. All rights reserved.)
    • Contributed Indexing:
      Keywords: Bovine leukemia virus; Genetic variation; Molecular clone; Proviral load; Reverse genetics; Transmission
    • Publication Date:
      Date Created: 20190825 Date Completed: 20200611 Latest Revision: 20200611
    • Publication Date:
      20221213
    • Accession Number:
      10.1016/j.virol.2019.08.015
    • Accession Number:
      31445323