AtNSE1 and AtNSE3 are required for embryo pattern formation and maintenance of cell viability during Arabidopsis embryogenesis.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Oxford University Press Country of Publication: England NLM ID: 9882906 Publication Model: Print Cited Medium: Internet ISSN: 1460-2431 (Electronic) Linking ISSN: 00220957 NLM ISO Abbreviation: J Exp Bot Subsets: MEDLINE
    • Publication Information:
      Original Publication: Oxford, UK : Oxford University Press,
    • Subject Terms:
      Body Patterning*; Arabidopsis/*embryology ; Arabidopsis/*metabolism ; Arabidopsis Proteins/*metabolism ; Nuclear Proteins/*metabolism ; Seeds/*cytology ; Seeds/*embryology ; Ubiquitin-Protein Ligases/*metabolism; Apoptosis ; Arabidopsis/cytology ; Arabidopsis/genetics ; Arabidopsis Proteins/chemistry ; Biological Transport ; Cell Survival ; Gene Expression Regulation, Plant ; Indoleacetic Acids/metabolism ; Meristem/metabolism ; Mutant Proteins/metabolism ; Mutation/genetics ; Nuclear Proteins/chemistry ; Phenotype ; Protein Binding ; Protein Domains ; Protein Structure, Secondary ; Seeds/ultrastructure ; Ubiquitin-Protein Ligases/chemistry
    • Abstract:
      Embryogenesis is an essential process during seed development in higher plants. It has previously been shown that mutation of the Arabidopsis non-SMC element genes AtNSE1 or AtNSE3 leads to early embryo abortion, and their proteins can interact with each other directly. However, the crucial regions of these proteins in this interaction and how the proteins are cytologically involved in Arabidopsis embryo development are unknown. In this study, we found that the C-terminal including the Ring-like motif of AtNSE1 can interact with the N-terminal of AtNSE3, and only the Ring-like motif is essential for binding with three α motifs of AtNSE2 (homologous to AtMMS21). Using genetic assays and by analysing molecular markers of cell fate decisions (STM, WOX5, and WOX8) in mutant nse1 and nse3 embryos, we found that AtNSE1 and AtNSE3 work non-redundantly in early embryo development, and that differentiation of the apical meristem and the hypophysis fails in the mutants, which have disrupted auxin transportation and responses. However, the upper cells of the suspensor in the mutants seem to have proper embryo cell identity. Cytological examination showed that cell death occurred from the early embryo stage, and that vacuolar programmed cell death and necrosis in the nse1 and nse3 mutant embryos led to ovule abortion. Thus, AtNSE1 and AtNSE3 are essential for maintaining cell viability and growth during early embryogenesis. Our results improve our understanding of the functions of SMC5/6 complex in early embryogenesis in Arabidopsis.
      (© The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology.)
    • References:
      Curr Biol. 2012 Sep 11;22(17):1564-75. (PMID: 22771042)
      J Cell Biol. 2013 Dec 23;203(6):917-27. (PMID: 24344187)
      Nat Struct Mol Biol. 2007 Jul;14(7):581-90. (PMID: 17589526)
      Mol Cell. 2009 Sep 11;35(5):657-68. (PMID: 19748359)
      J Biol Chem. 2003 Nov 14;278(46):45460-7. (PMID: 12966087)
      Curr Biol. 2013 Mar 4;23(5):362-71. (PMID: 23394827)
      Plant Signal Behav. 2008 Jul;3(7):469-70. (PMID: 19704488)
      Genes Dev. 2004 Aug 15;18(16):1964-9. (PMID: 15314023)
      Genes Dev. 2005 Jun 1;19(11):1269-87. (PMID: 15937217)
      Development. 2017 May 1;144(9):1648-1660. (PMID: 28302748)
      EMBO J. 2000 Apr 3;19(7):1691-702. (PMID: 10747036)
      Development. 2002 Jul;129(13):3195-206. (PMID: 12070094)
      Development. 1998 Aug;125(16):3027-35. (PMID: 9671577)
      Annu Rev Plant Biol. 2012;63:483-506. (PMID: 22224452)
      EMBO J. 2017 May 2;36(9):1261-1278. (PMID: 28320736)
      Proc Natl Acad Sci U S A. 2005 Mar 29;102(13):4777-82. (PMID: 15738391)
      Chromosome Res. 2009;17(2):251-63. (PMID: 19308705)
      Science. 2008 Oct 24;322(5901):594-7. (PMID: 18948541)
      Mol Biol Cell. 2004 Nov;15(11):4866-76. (PMID: 15331764)
      Cold Spring Harb Perspect Biol. 2009 Nov;1(5):a001545. (PMID: 20066117)
      Nature. 1996 Jan 4;379(6560):66-9. (PMID: 8538741)
      Curr Biol. 2005 Nov 8;15(21):1899-911. (PMID: 16271866)
      Genes Dev. 2002 Jul 1;16(13):1610-5. (PMID: 12101120)
      Dev Cell. 2015 Jul 27;34(2):242-51. (PMID: 26166301)
      Plant Cell. 2016 Sep;28(9):2225-2237. (PMID: 27492969)
      Biol Open. 2016 Jul 15;5(7):928-41. (PMID: 27288507)
      Plant Cell. 1993 Oct;5(10):1371-1381. (PMID: 12271036)
      Trends Plant Sci. 2010 Jan;15(1):23-30. (PMID: 19963427)
      Dev Growth Differ. 2015 Aug;57(6):430-43. (PMID: 26036668)
      Mol Cell Biol. 2005 Jan;25(1):172-84. (PMID: 15601840)
      PLoS One. 2013;8(3):e59866. (PMID: 23555814)
      Mol Cell Biol. 2005 Jan;25(1):185-96. (PMID: 15601841)
      J Exp Bot. 2015 Apr;66(7):2079-91. (PMID: 25871650)
      J Exp Bot. 2017 Feb 1;68(5):1039-1054. (PMID: 28207059)
      Dev Cell. 2011 Feb 15;20(2):264-70. (PMID: 21316593)
      C R Biol. 2008 Oct;331(10):715-25. (PMID: 18926485)
      Nature. 2007 Apr 12;446(7137):811-4. (PMID: 17429400)
      Mol Cell Biol. 2005 Aug;25(16):7021-32. (PMID: 16055714)
      EMBO J. 1998 Mar 2;17(5):1405-11. (PMID: 9482737)
      Plant Cell. 2009 Sep;21(9):2688-99. (PMID: 19737979)
      Cell Death Differ. 2011 Aug;18(8):1241-6. (PMID: 21494263)
      Genes (Basel). 2018 Jan 12;9(1):null. (PMID: 29329249)
      Nat Cell Biol. 2011 May;13(5):611-5. (PMID: 21478855)
      Development. 2004 Feb;131(3):657-68. (PMID: 14711878)
      Proc Natl Acad Sci U S A. 2015 Oct 6;112(40):12432-7. (PMID: 26396256)
      Plant Physiol. 2003 Dec;133(4):1882-92. (PMID: 14605218)
      Nature. 2003 Nov 13;426(6963):147-53. (PMID: 14614497)
      Sex Plant Reprod. 2011 Jun;24(2):161-9. (PMID: 21225434)
      Nature. 2005 Jan 6;433(7021):39-44. (PMID: 15635403)
      BMC Plant Biol. 2012 Aug 01;12:126. (PMID: 22853005)
      Dev Biol. 2007 Sep 15;309(2):306-16. (PMID: 17706632)
      EMBO Rep. 2013 Sep;14(9):817-22. (PMID: 23907539)
      Proc Natl Acad Sci U S A. 2018 Apr 17;115(16):E3837-E3845. (PMID: 29610335)
      Mol Biol Cell. 2012 Apr;23(8):1558-68. (PMID: 22357613)
      Development. 2015 Feb 1;142(3):420-30. (PMID: 25605778)
      Curr Biol. 2009 Jun 9;19(11):909-14. (PMID: 19398337)
      Front Plant Sci. 2014 Aug 15;5:393. (PMID: 25177324)
      Mol Cell Biol. 2008 Feb;28(4):1197-206. (PMID: 18086888)
      Plant Physiol. 2013 Apr;161(4):1755-68. (PMID: 23426194)
      Annu Rev Cell Dev Biol. 2007;23:207-36. (PMID: 17539754)
      Mol Biol Cell. 2008 Oct;19(10):4099-109. (PMID: 18667531)
    • Contributed Indexing:
      Keywords: AtNSE1; AtNSE3; Arabidopsis; embryo; pattern formation; programmed cell death
    • Accession Number:
      0 (AT1G34770 protein, Arabidopsis)
      0 (Arabidopsis Proteins)
      0 (Indoleacetic Acids)
      0 (Mutant Proteins)
      0 (Nuclear Proteins)
      EC 2.3.2.27 (AT5G21140 protein, Arabidopsis)
      EC 2.3.2.27 (Ubiquitin-Protein Ligases)
    • Publication Date:
      Date Created: 20190814 Date Completed: 20200810 Latest Revision: 20201008
    • Publication Date:
      20240829
    • Accession Number:
      PMC6859727
    • Accession Number:
      10.1093/jxb/erz373
    • Accession Number:
      31408172