Molecular characterization of novel mitochondrial peroxiredoxins from the Antarctic emerald rockcod and their gene expression in response to environmental warming.

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  • Additional Information
    • Source:
      Publisher: Elsevier Science Country of Publication: United States NLM ID: 100959500 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-0456 (Print) Linking ISSN: 15320456 NLM ISO Abbreviation: Comp Biochem Physiol C Toxicol Pharmacol Subsets: MEDLINE
    • Publication Information:
      Original Publication: New York, NY : Elsevier Science, c2000-
    • Subject Terms:
      Peroxiredoxins*/classification ; Peroxiredoxins*/metabolism; Mitochondria/*enzymology ; Perciformes/*metabolism; Amino Acid Sequence ; Animals ; Antarctic Regions ; Fish Proteins/classification ; Fish Proteins/metabolism ; Gene Expression ; Global Warming ; Phylogeny ; Protein Isoforms ; Temperature
    • Abstract:
      In the present study we describe the molecular characterization of the two paralogous mitochondrial peroxiredoxins from Trematomus bernacchii, a teleost that plays a pivotal role in the Antarctic food chain. The two putative amino acid sequences were compared with orthologs from other fish, highlighting a high percentage of identity and similarity with the respective variant, in particular for the residues that are essential for the characteristic peroxidase activity of these enzymes. The temporal expression of Prdx3 and Prdx5 mRNAs in response to short-term thermal stress showed a general upregulation of prdx3, suggesting that this isoform is the most affected by temperature increase. These data, together with the peculiar differences between the molecular structures of the two mitochondrial Prdxs in T. bernacchii as well as in the tropical species Stegastes partitus, suggest an adaptation that allowed these poikilothermic aquatic vertebrates to colonize very different environments, characterized by different temperature ranges.
      (Copyright © 2019 Elsevier Inc. All rights reserved.)
    • Contributed Indexing:
      Keywords: Antarctica; Fish; Gene expression; Global warming; Molecular evolution; Peroxiredoxins
    • Accession Number:
      0 (Fish Proteins)
      0 (Protein Isoforms)
      EC 1.11.1.15 (Peroxiredoxins)
    • Publication Date:
      Date Created: 20190803 Date Completed: 20200221 Latest Revision: 20200221
    • Publication Date:
      20240829
    • Accession Number:
      10.1016/j.cbpc.2019.108580
    • Accession Number:
      31374295