TLR2 Promotes Monocyte/Macrophage Recruitment Into the Liver and Microabscess Formation to Limit the Spread of Listeria Monocytogenes .

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  • Additional Information
    • Source:
      Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101560960 Publication Model: eCollection Cited Medium: Internet ISSN: 1664-3224 (Electronic) Linking ISSN: 16643224 NLM ISO Abbreviation: Front Immunol Subsets: MEDLINE
    • Publication Information:
      Original Publication: [Lausanne : Frontiers Research Foundation]
    • Subject Terms:
      Listeria monocytogenes/*immunology ; Listeriosis/*immunology ; Liver/*immunology ; Liver Abscess/*immunology ; Macrophages/*immunology ; Monocytes/*immunology ; Toll-Like Receptor 2/*immunology; Animals ; Listeriosis/genetics ; Listeriosis/microbiology ; Listeriosis/pathology ; Liver/microbiology ; Liver/pathology ; Liver Abscess/genetics ; Liver Abscess/microbiology ; Liver Abscess/pathology ; Macrophages/pathology ; Mice ; Mice, Knockout ; Monocytes/pathology ; Toll-Like Receptor 2/genetics
    • Abstract:
      TLR2 signaling plays a critical protective role against acute Listeria monocytogenes (Lm) infection by up-regulating inflammatory cytokines and promoting macrophage antimicrobial capabilities. However, the underlying mechanism by which TLR2 regulates hepatic macrophage-mediated anti-Lm immune responses remains poorly understood. In this study, we found that both the absolute number and proportion of monocyte/macrophage (Mo/MΦ) in the liver and spleen of Tlr2 -/- mice were significantly lower compared to wild type mice. Changes in TLR2 signaling in both hepatocytes and Mo/MΦs were associated with the infiltration of Mo/MΦs in response to Lm-infection. Analyses by proteome profiler array and ELISA revealed that hepatocytes recruited Mo/MΦs via TLR2-dependent secretion of CCL2 and CXCL1, which was confirmed by receptor blocking and exogenous chemokine administration. Importantly, we found that TLR2 contributed to macrophage mobility in the liver through a TLR2/NO/F-actin pathway, facilitating the formation of macrophage-associated hepatic microabscesses. Moreover, TLR2 activation induced the expression of several PRRs on hepatic macrophages associated with the recognition of Lm and augmented macrophage bacterial clearance activity. Our findings provide insight into the intrinsic mechanisms of TLR2-induced Mo/MΦ migration and mobility, as well as the interaction between macrophages and hepatocytes in resistance to Lm infection.
    • References:
      Pathol Int. 1999 Jun;49(6):519-32. (PMID: 10469395)
      Nat Med. 2000 May;6(5):573-7. (PMID: 10802715)
      J Immunol. 2002 Oct 1;169(7):3869-75. (PMID: 12244184)
      J Neurochem. 2002 Nov;83(3):490-503. (PMID: 12390511)
      Infect Immun. 2004 Apr;72(4):2131-9. (PMID: 15039335)
      Infect Immun. 2005 Aug;73(8):5144-51. (PMID: 16041031)
      Nat Immunol. 2006 Mar;7(3):311-7. (PMID: 16462739)
      Hepatology. 2007 Sep;46(3):706-15. (PMID: 17626270)
      J Infect Dis. 2008 Jul 1;198(1):115-24. (PMID: 18462086)
      Microbes Infect. 2008 Jul;10(9):1041-50. (PMID: 18775788)
      Int J Cancer. 2009 Apr 15;124(8):1785-93. (PMID: 19058221)
      Free Radic Biol Med. 2009 Sep 15;47(6):741-9. (PMID: 19523512)
      Inflamm Allergy Drug Targets. 2009 Sep;8(4):307-18. (PMID: 19534673)
      Biochem Biophys Res Commun. 2009 Sep 4;386(4):744-9. (PMID: 19559671)
      J Aerosol Med Pulm Drug Deliv. 2010 Aug;23(4):207-17. (PMID: 20109124)
      Infect Immun. 2010 Jun;78(6):2857-67. (PMID: 20368346)
      J Immunol. 2010 Jun 1;184(11):6266-74. (PMID: 20435926)
      Mol Syst Biol. 2010 Jun 8;6:371. (PMID: 20531401)
      Trends Immunol. 2011 Oct;32(10):470-7. (PMID: 21664185)
      J Cell Biochem. 2012 Jan;113(1):80-92. (PMID: 21898535)
      Adv Immunol. 2012;113:119-34. (PMID: 22244581)
      Adv Immunol. 2012;113:157-74. (PMID: 22244583)
      Vet Res. 2012 May 25;43:47. (PMID: 22630829)
      Sci Rep. 2012;2:786. (PMID: 23139859)
      PLoS Pathog. 2013;9(4):e1003291. (PMID: 23593001)
      Matrix Biol. 2014 Apr;35:132-42. (PMID: 24361484)
      Nat Rev Immunol. 2014 Mar;14(3):166-80. (PMID: 24481336)
      Immunogenetics. 2014 Apr;66(4):231-42. (PMID: 24566892)
      Immunity. 2015 Jan 20;42(1):145-58. (PMID: 25577440)
      Immunity. 2015 Jan 20;42(1):10-2. (PMID: 25607453)
      Infect Immun. 2015 Apr;83(4):1641-9. (PMID: 25667264)
      Trends Cell Biol. 2015 Jun;25(6):330-8. (PMID: 25670529)
      PLoS One. 2015 Mar 23;10(3):e0121551. (PMID: 25799230)
      Semin Immunopathol. 2015 May;37(3):301-10. (PMID: 25860798)
      Arthritis Res Ther. 2015 Jun 09;17:153. (PMID: 26055925)
      J Appl Microbiol. 2016 Feb;120(2):251-65. (PMID: 26509460)
      Sci Rep. 2015 Nov 27;5:17370. (PMID: 26610705)
      Cell Mol Immunol. 2016 May;13(3):301-15. (PMID: 26685902)
      Cell Mol Immunol. 2016 May;13(3):316-27. (PMID: 26908374)
      Oncoimmunology. 2015 Aug 12;5(2):e1074376. (PMID: 27057441)
      Immunol Cell Biol. 2017 May;95(5):424-435. (PMID: 27826145)
      J Hepatol. 2017 Jun;66(6):1300-1312. (PMID: 28267621)
      Sci Rep. 2017 Jun 21;7(1):3952. (PMID: 28638086)
      Eur J Immunol. 2018 Sep;48(9):1470-1480. (PMID: 29935120)
    • Contributed Indexing:
      Keywords: Listeria monocytogenes; TLR2; hepatocyte; macrophage; migration
    • Accession Number:
      0 (Tlr2 protein, mouse)
      0 (Toll-Like Receptor 2)
    • Publication Date:
      Date Created: 20190713 Date Completed: 20201015 Latest Revision: 20201015
    • Publication Date:
      20221213
    • Accession Number:
      PMC6607897
    • Accession Number:
      10.3389/fimmu.2019.01388
    • Accession Number:
      31297109