Temperature synchronization of the Drosophila circadian clock protein PERIOD is controlled by the TRPA channel PYREXIA.

Publisher: Nature Publishing Group UK Country of Publication: England NLM ID: 101719179 Publication Model: eCollection Cited Medium: Internet ISSN: 2399-3642 (Electronic) Linking ISSN: 23993642 NLM ISO Abbreviation: Commun Biol

Original Publication: London, United Kingdom : Nature Publishing Group UK, [2018]-

Circadian Clocks*; Brain/*physiology ; Calcium/*physiology ; Drosophila Proteins/*physiology ; Drosophila melanogaster/*physiology ; Period Circadian Proteins/*physiology ; Transient Receptor Potential Channels/*physiology; Animals ; Animals, Genetically Modified ; Circadian Rhythm ; Crosses, Genetic ; Drosophila Proteins/genetics ; Female ; Genotype ; Light ; Male ; Mutation ; Neurons/physiology ; Oscillometry ; Period Circadian Proteins/genetics ; Temperature ; Transcription Factors/physiology ; Transient Receptor Potential Channels/genetics

Circadian clocks are endogenous molecular oscillators that temporally organize behavioral activity thereby contributing to the fitness of organisms. To synchronize the fly circadian clock with the daily fluctuations of light and temperature, these environmental cues are sensed both via brain clock neurons, and by light and temperature sensors located in the peripheral nervous system. Here we demonstrate that the TRPA channel PYREXIA (PYX) is required for temperature synchronization of the key circadian clock protein PERIOD. We observe a molecular synchronization defect explaining the previously reported defects of pyx mutants in behavioral temperature synchronization. Surprisingly, surgical ablation of pyx -mutant antennae partially rescues behavioral synchronization, indicating that antennal temperature signals are modulated by PYX function to synchronize clock neurons in the brain. Our results suggest that PYX protects antennal neurons from faulty signaling that would otherwise interfere with temperature synchronization of the circadian clock neurons in the brain.
Competing Interests: Competing interestsThe authors declare no competing interests.

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Keywords: Circadian rhythms and sleep; Ion channels in the nervous system

0 (Drosophila Proteins)
0 (PER protein, Drosophila)
0 (Period Circadian Proteins)
0 (Transcription Factors)
0 (Transient Receptor Potential Channels)
0 (pyx protein, Drosophila)
SY7Q814VUP (Calcium)

Date Created: 20190710 Date Completed: 20200501 Latest Revision: 20240229

20240229

PMC6602953

10.1038/s42003-019-0497-0

31286063