Protective Effect of Schisandra chinensis Polysaccharides Against the Immunological Liver Injury in Mice Based on Nrf2/ARE and TLR4/NF- κ B Signaling Pathway.

Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 9812512 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1557-7600 (Electronic) Linking ISSN: 1096620X NLM ISO Abbreviation: J Med Food Subsets: MEDLINE

Original Publication: Larchmont, NY : Mary Ann Liebert, Inc., c1998-

Drugs, Chinese Herbal/*administration & dosage ; Liver/*injuries ; Liver Diseases/*prevention & control ; NF-E2-Related Factor 2/*immunology ; NF-kappa B/*immunology ; Polysaccharides/*administration & dosage ; Protective Agents/*administration & dosage ; Schisandra/*chemistry ; Toll-Like Receptor 4/*immunology; Animals ; Humans ; Interleukin-1beta/genetics ; Interleukin-1beta/immunology ; Interleukin-6/genetics ; Interleukin-6/immunology ; Kelch-Like ECH-Associated Protein 1/genetics ; Kelch-Like ECH-Associated Protein 1/immunology ; Liver/drug effects ; Liver/immunology ; Liver Diseases/genetics ; Liver Diseases/immunology ; Male ; Mice ; Mice, Inbred ICR ; NF-E2-Related Factor 2/genetics ; NF-kappa B/genetics ; Nitric Oxide/immunology ; Signal Transduction/drug effects ; Toll-Like Receptor 4/genetics ; Tumor Necrosis Factor-alpha/genetics ; Tumor Necrosis Factor-alpha/immunology

We have previously demonstrated the hepatoprotective effect of Schisandra chinensis polysaccharides (SCP) against the liver injury induced by alcohol, high-fat diet, and carbon tetrachloride in mice. In this study, we investigated the effect of SCP against the immunological liver injury induced by concanavalin A (Con A) in mice. The results showed that SCP could significantly reduce the level of alanine aminotransferase (ALT), aspartate aminotransferase (AST), tumor necrosis factor- α (TNF- α ), interferon- γ (IFN- γ ), interleukin-1 β (IL-1 β ), and interleukin-6 (IL-6) in the serum of mice with immunological liver injury. SCP could significantly decrease the content of malondialdehyde (MDA) and nitric oxide (NO) and increase the activity of superoxide dismutase (SOD) and glutathione (GSH) in the liver tissue. SCP could significantly increase the number of CD4 ⁺ and decrease the number of CD8 ⁺ in the peripheral blood, and elevate the ratio of CD4 ⁺ /CD8 ⁺ . SCP could significantly downregulate the expression of Kelch-like ECH-associated protein 1 (Keap1) and upregulate the expression of nuclear factor-erythroid 2-related factor2 (Nrf2) and downstream gene heme oxygenase-1 (HO-1), and downregulate the expression of toll-like receptor 4 (TLR4), myeloid differentiation primary response gene 88 (MyD88), and nuclear factor-kappa B (NF- κ B) proteins. This study indicates that SCP can reduce the release of a large number of inflammatory factors to inhibit the oxidative stress in mice with the immunological liver injury induced by Con A, and its mechanism is closely related to the regulation of Nrf2/antioxidant response element and TLR4/NF- κ B signaling pathways.

Keywords: anti-inflammatory; antioxidant; concanavalin A; hepatoprotection

0 (Drugs, Chinese Herbal)
0 (Interleukin-1beta)
0 (Interleukin-6)
0 (Kelch-Like ECH-Associated Protein 1)
0 (NF-E2-Related Factor 2)
0 (NF-kappa B)
0 (Polysaccharides)
0 (Protective Agents)
0 (Toll-Like Receptor 4)
0 (Tumor Necrosis Factor-alpha)
31C4KY9ESH (Nitric Oxide)

Date Created: 20190706 Date Completed: 20200131 Latest Revision: 20200131

20221213

10.1089/jmf.2018.4377

31274380