Expression and molecular characterization of stress-responsive genes (hsp70 and Mn-sod) and evaluation of antioxidant enzymes (CAT and GPx) in heavy metal exposed freshwater ciliate, Tetmemena sp.

Publisher: Reidel Country of Publication: Netherlands NLM ID: 0403234 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-4978 (Electronic) Linking ISSN: 03014851 NLM ISO Abbreviation: Mol Biol Rep Subsets: MEDLINE

Original Publication: Dordrecht, Boston, Reidel.

Ciliophora/*metabolism ; HSP70 Heat-Shock Proteins/*metabolism ; Superoxide Dismutase/*metabolism; Antioxidants/metabolism ; Antioxidants/physiology ; Cadmium/adverse effects ; Cadmium/pharmacology ; Catalase/analysis ; Catalase/metabolism ; Ciliophora/genetics ; Copper/adverse effects ; Copper/pharmacology ; Ecosystem ; Fresh Water ; Gene Expression Regulation, Bacterial/genetics ; Glutathione Peroxidase/analysis ; Glutathione Peroxidase/metabolism ; HSP70 Heat-Shock Proteins/genetics ; Metals, Heavy/adverse effects ; Oxidative Stress/drug effects ; Stress, Physiological/genetics ; Superoxide Dismutase/genetics ; Water Pollutants, Chemical/toxicity

Response of heavy metals namely cadmium (Cd) and copper (Cu) on the expression of stress responsive genes in the fresh water ciliate, Tetmemena sp. (single cell eukaryote) was studied. Stress responsive genes include heat shock protein genes and genes involved in antioxidant defence system. Quantitative real time PCR (qRT-PCR) was employed to evaluate the effects of Cd and Cu on the expression of cytosolic hsp70 and Mn-sod genes. Increase in the expression of these genes was observed after exposure with the heavy metals. The macronuclear cytosolic hsp70 and Mn-sod (SOD2) genes were also sequenced and characterized using various bioinformatics tools. In antioxidant defence system, the superoxide dismutase (SOD) family is a first line antioxidant enzyme group involved in catalysing reactive oxygen species (ROS) to hydrogen peroxide and molecular oxygen. Influence of Cd and Cu on the activity of SOD has already been reported by our group. Therefore, the enzymatic activities of antioxidant enzymes, catalase (CAT) and glutathione peroxidase (GPx) were studied in the presence of Cd and Cu and there was significant increase in activity of these enzymes in concentration dependent manner. This study suggests that cytosolic hsp70, Mn-sod and the antioxidant enzymes such as CAT and GPx can be used as effective molecular biomarkers for heavy metal toxicity and Tetmemena sp. can be used as potential model for understanding the molecular response to heavy metal contamination in aquatic ecosystems.

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Keywords: Antioxidant enzymes; Biomarkers; Freshwater ciliate; Heavy metal stress; Stress genes; Tetmemena sp.

0 (Antioxidants)
0 (HSP70 Heat-Shock Proteins)
0 (Metals, Heavy)
0 (Water Pollutants, Chemical)
00BH33GNGH (Cadmium)
789U1901C5 (Copper)
EC 1.11.1.6 (Catalase)
EC 1.11.1.9 (Glutathione Peroxidase)
EC 1.15.1.1 (Superoxide Dismutase)

Date Created: 20190706 Date Completed: 20200309 Latest Revision: 20220125

20221213

10.1007/s11033-019-04942-0

31273612