AIE Nanoassemblies for Discrimination of Glycosaminoglycans and Heparin Quality Control.

Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6882 (Electronic) Linking ISSN: 00032700 NLM ISO Abbreviation: Anal Chem Subsets: MEDLINE

Original Publication: Washington, American Chemical Society.

Quality Control*; Biosensing Techniques/*methods ; Glycosaminoglycans/*blood ; Heparin/*blood ; Nanocomposites/*chemistry ; Polymers/*chemistry; Glycosaminoglycans/chemistry ; Heparin/chemistry ; Humans

The discrimination of glycosaminoglycans (GAGs) is a challenging task but of great importance to ensure their safe use in clinics. Herein, four supramolecular AIE nanoassemblies denoted as PDDA-TPE 100 , PDDA-TPE 75 , PDDA-TPE 50 , and PDDA-TPE 25 were synthesized by loading different amounts of the negatively charged AIEgen TPE onto the surface of the positively charged polymer PDDA . These AIE nanoassemblies were utilized for the construction of a fluorescent sensor array, which was able to discriminate various GAGs on the basis of a compaction to displacement reaction mechanism. LDA and HCA results revealed that GAGs including Hep, Chs, HA, CTS, DS, and OSCS can be discriminated with 100% accuracy. The four-sensor array was then simplified to a three-sensor array using PDDA-TPE 100 , PDDA-TPE 75 , and PDDA-TPE 50 as the sensors, which was determined to be still powerful in discrimination of various GAGs, accurate identification of unknown GAG samples, sensitive detection of trace OSCS contaminant in Hep, and identification of Hep and other biologically abundant anions. Moreover, the three-sensor array was even successfully applied for differentiating GAGs in serum media.

0 (Glycosaminoglycans)
0 (Polymers)
9005-49-6 (Heparin)

Date Created: 20190628 Date Completed: 20200921 Latest Revision: 20200921

20240829

10.1021/acs.analchem.9b02516

31246423