Diminished secretion and function of IL-29 is associated with impaired IFN-α response of neonatal plasmacytoid dendritic cells.

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  • Additional Information
    • Source:
      Publisher: Oxford University Press Country of Publication: England NLM ID: 8405628 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1938-3673 (Electronic) Linking ISSN: 07415400 NLM ISO Abbreviation: J Leukoc Biol Subsets: MEDLINE
    • Publication Information:
      Publication: 2023- : Oxford : Oxford University Press
      Original Publication: New York : Alan R. Liss, c1984-
    • Subject Terms:
      Dendritic Cells/*immunology ; Interferon-alpha/*immunology ; Interferons/*immunology ; Interleukins/*immunology ; Oligodeoxyribonucleotides/*pharmacology; Adolescent ; Adult ; Dendritic Cells/cytology ; Female ; Humans ; Infant, Newborn ; Interferon Regulatory Factor-7/immunology ; Interleukin-10/immunology ; Male ; Receptors, Interferon/immunology ; STAT1 Transcription Factor/immunology ; Toll-Like Receptor 9/agonists ; Toll-Like Receptor 9/immunology
    • Abstract:
      Plasmacytoid dendritic cells (pDCs) are key players in the antiviral immune response and type III IFNs such as IL-29 appear to play a pivotal role in pDC function. Pronounced susceptibility to viral infections in neonates is partly resulting from diminished antiviral immune mechanisms. Accordingly, the aim of the present study was to investigate the impact of IL-29 in the altered immune response of neonatal pDCs. PBMCs of adult and term newborns were stimulated with CpG-ODN2216 in the presence or absence of IL-29 and assessed for IFN-α production, downstream-signaling, and activation marker expression. A significantly lower IL-29 production after TLR9-specific stimulation was demonstrated in neonatal pDCs. IL-29 enhanced the IFN-α production of pDCs in adults compared to newborns. Newborn pDCs displayed a significantly lower surface expression of IL-10 and IL-28Rα receptor resulting in diminished STAT1 and IRF7 activation. Interestingly, concomitant stimulation with CpG-ODN2216/IL-29 had no impact on the expression of surface activation and maturation markers of pDCs in neither population. The diminished antiviral immune response of neonatal pDCs is associated with reduced production and cellular responses toward IL-29. Potential therapeutic agents enhancing the IL-29 response in neonatal pDCs possibly augment viral protection in newborns.
      (© 2019 The Authors. Journal of Leukocyte Biology published by Wiley Periodicals, Inc. on behalf of Society for Leukocyte Biology.)
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    • Contributed Indexing:
      Keywords: interleukin-29; neonatal Immunity; plasmacytoid dendritic cells; toll-like receptor; type III interferons
    • Accession Number:
      0 (CpG ODN 2216)
      0 (interferon-lambda, human)
      0 (IFNLR1 protein, human)
      0 (IL10 protein, human)
      0 (IRF7 protein, human)
      0 (Interferon Regulatory Factor-7)
      0 (Interferon-alpha)
      0 (Interleukins)
      0 (Oligodeoxyribonucleotides)
      0 (Receptors, Interferon)
      0 (STAT1 Transcription Factor)
      0 (STAT1 protein, human)
      0 (TLR9 protein, human)
      0 (Toll-Like Receptor 9)
      130068-27-8 (Interleukin-10)
      9008-11-1 (Interferons)
    • Publication Date:
      Date Created: 20190619 Date Completed: 20200527 Latest Revision: 20231213
    • Publication Date:
      20231215
    • Accession Number:
      PMC6852569
    • Accession Number:
      10.1002/JLB.4A0518-189R
    • Accession Number:
      31211458