The cytoplasmic portion of the T3SS inner membrane ring components sort into distinct families based on biophysical properties.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101731734 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-1454 (Electronic) Linking ISSN: 15709639 NLM ISO Abbreviation: Biochim Biophys Acta Proteins Proteom Subsets: MEDLINE
    • Publication Information:
      Original Publication: Amsterdam : Elsevier
    • Subject Terms:
      Bacteria*/chemistry ; Bacteria*/metabolism ; Bacterial Proteins*/chemistry ; Bacterial Proteins*/metabolism ; Cell Membrane*/chemistry ; Cell Membrane*/metabolism ; Type III Secretion Systems*/chemistry ; Type III Secretion Systems*/metabolism
    • Abstract:
      Type III secretion systems are used by many Gram-negative bacteria to inject effector proteins into eukaryotic cells to subvert their normal activities. Structurally conserved portions of the type III secretion apparatus include a: basal body located within the bacterial envelope; an exposed needle with tip complex that delivers effectors across the target cell membrane; and cytoplasmic sorting platform that selects cargo and powers secretion. While structurally conserved, the individual proteins that make up this nanomachine are typically not interchangeable though they do tend to fall into families. Here we selected a single domain from the inner membrane ring of the basal body from six different type III secretion systems (called SctD using a proposed unifying nomenclature). The selected domain creates an integral interface between the basal body and the sorting platform. Therefore, it represents a pivotal point between two distinct assemblies. All six protein domains possess a structural motif called a forkhead-associated-like (FHA-like) domain but differ greatly in their sequences and solution behaviors. These differences are used here to define family boundaries for these FHA-like domains. The data parallel, though not precisely, family boundaries defined by other proteins within the apparatus and by phylogenetic analysis. Ultimately, differences in the families are likely to reflect differences in the activities of these type III secretion systems or the host niches in which these pathogens are found.
      (Copyright © 2019 Elsevier B.V. All rights reserved.)
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    • Grant Information:
      P30 GM110761 United States GM NIGMS NIH HHS; R01 AI123351 United States AI NIAID NIH HHS
    • Contributed Indexing:
      Keywords: Protein stability; SctD; Type III secretion system
    • Accession Number:
      0 (Bacterial Proteins)
      0 (Type III Secretion Systems)
    • Publication Date:
      Date Created: 20190614 Date Completed: 20191126 Latest Revision: 20200901
    • Publication Date:
      20221213
    • Accession Number:
      PMC6626570
    • Accession Number:
      10.1016/j.bbapap.2019.06.002
    • Accession Number:
      31195141