Item request has been placed!
×
Item request cannot be made.
×
Processing Request
A high-density BAC physical map covering the entire MHC region of addax antelope genome.
Item request has been placed!
×
Item request cannot be made.
×
Processing Request
- Additional Information
- Source:
Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2164 (Electronic) Linking ISSN: 14712164 NLM ISO Abbreviation: BMC Genomics Subsets: MEDLINE
- Publication Information:
Original Publication: London : BioMed Central, [2000-
- Subject Terms:
- Abstract:
Background: The mammalian major histocompatibility complex (MHC) harbours clusters of genes associated with the immunological defence of animals against infectious pathogens. At present, no complete MHC physical map is available for any of the wild ruminant species in the world.
Results: The high-density physical map is composed of two contigs of 47 overlapping bacterial artificial chromosome (BAC) clones, with an average of 115 Kb for each BAC, covering the entire addax MHC genome. The first contig has 40 overlapping BAC clones covering an approximately 2.9 Mb region of MHC class I, class III, and class IIa, and the second contig has 7 BAC clones covering an approximately 500 Kb genomic region that harbours MHC class IIb. The relative position of each BAC corresponding to the MHC sequence was determined by comparative mapping using PCR screening of the BAC library of 192,000 clones, and the order of BACs was determined by DNA fingerprinting. The overlaps of neighboring BACs were cross-verified by both BAC-end sequencing and co-amplification of identical PCR fragments within the overlapped region, with their identities further confirmed by DNA sequencing.
Conclusions: We report here the successful construction of a high-quality physical map for the addax MHC region using BACs and comparative mapping. The addax MHC physical map we constructed showed one gap of approximately 18 Mb formed by an ancient autosomal inversion that divided the MHC class II into IIa and IIb. The autosomal inversion provides compelling evidence that the MHC organizations in all of the ruminant species are relatively conserved.
- References:
Annu Rev Immunol. 2018 Apr 26;36:383-409. (PMID: 29677478)
Nat Rev Immunol. 2011 Nov 11;11(12):823-36. (PMID: 22076556)
Biol Conserv. 2010 Mar;143(3):537-544. (PMID: 32226082)
Sci Rep. 2016 Mar 02;6:22471. (PMID: 26932528)
Cell Mol Immunol. 2013 Jul;10(4):292-302. (PMID: 23604044)
Science. 2009 Apr 24;324(5926):522-8. (PMID: 19390049)
Immunogenetics. 2013 Oct;65(10):749-62. (PMID: 23925440)
Zoo Biol. 2012 Jan-Feb;31(1):98-106. (PMID: 21898577)
Front Immunol. 2017 Mar 15;8:282. (PMID: 28360911)
Proc Natl Acad Sci U S A. 2011 Mar 8;108(10):4012-7. (PMID: 21300875)
J Virol. 2016 Jul 11;90(15):6625-6641. (PMID: 27170754)
Mamm Genome. 1999 Jun;10(6):585-7. (PMID: 10341090)
Immunol Rev. 2010 Mar;234(1):305-16. (PMID: 20193027)
Nature. 2017 Nov 23;551(7681):525-528. (PMID: 29107940)
Infect Genet Evol. 2016 Oct;44:210-217. (PMID: 27423515)
Nature. 1983 Apr 14;302(5909):575-81. (PMID: 6300689)
Science. 2014 Jun 6;344(6188):1168-1173. (PMID: 24904168)
BMC Genomics. 2009 Apr 24;10:182. (PMID: 19393056)
Comp Biochem Physiol A Mol Integr Physiol. 2008 Feb;149(2):142-9. (PMID: 18083600)
Gastroenterology. 1981 Jul;81(1):159-73. (PMID: 7016658)
Curr Opin Immunol. 2014 Feb;26:115-22. (PMID: 24463216)
Proc Natl Acad Sci U S A. 2011 May 17;108(20):8396-401. (PMID: 21536896)
Proc Natl Acad Sci U S A. 2005 Jun 14;102(24):8668-73. (PMID: 15939887)
Acta Trop. 2015 Dec;152:112-115. (PMID: 26314229)
Immunogenetics. 2005 Nov;57(10):763-74. (PMID: 16220348)
Genetics. 1951 May;36(3):306-10. (PMID: 14840651)
Anat Histol Embryol. 2017 Jun;46(3):282-293. (PMID: 28295511)
J Hered. 2007;98(5):390-9. (PMID: 17675392)
Immunogenetics. 1994;40(3):230-4. (PMID: 8039830)
Ecol Evol. 2017 Aug 17;7(19):7638-7649. (PMID: 29043021)
Zoo Biol. 2011 Jul-Aug;30(4):399-411. (PMID: 20853411)
BMC Genomics. 2012 Aug 16;13:398. (PMID: 22897909)
BMC Genomics. 2007 Sep 08;8:315. (PMID: 17825108)
BMC Genomics. 2008 Sep 11;9:409. (PMID: 18786271)
Cytometry A. 2005 May;65(1):26-34. (PMID: 15779063)
Nat Rev Genet. 2004 Dec;5(12):889-99. (PMID: 15573121)
Proc Biol Sci. 2005 Oct 7;272(1576):2009-16. (PMID: 16191610)
J Immunol. 1998 Sep 15;161(6):2985-93. (PMID: 9743362)
Proc Biol Sci. 1995 Jun 22;260(1359):245-9. (PMID: 7630893)
Sci Rep. 2017 Mar 31;7:45518. (PMID: 28361880)
Cytogenet Genome Res. 2003;102(1-4):189-95. (PMID: 14970701)
Immunogenetics. 2004 Feb;55(11):748-55. (PMID: 14745522)
Immunol Rev. 1999 Feb;167:145-58. (PMID: 10319257)
BMC Evol Biol. 2015 Apr 13;15:63. (PMID: 25886729)
Comput Appl Biosci. 1997 Oct;13(5):523-35. (PMID: 9367125)
Rev Sci Tech. 1998 Apr;17(1):108-20. (PMID: 9638804)
Genome Res. 1997 Nov;7(11):1072-84. (PMID: 9371743)
Immunogenetics. 2018 May;70(5):317-326. (PMID: 29063126)
Genome Biol. 2014;15(12):557. (PMID: 25496777)
- Grant Information:
153E11KYSB20130101 Chinese Academy of Sciences; 81871130 National Natural Science Foundation of China
- Contributed Indexing:
Keywords: Addax nasomaculatus; BAC; MHC; Physical map
- Accession Number:
0 (Histocompatibility Antigens Class I)
0 (Histocompatibility Antigens Class II)
- Publication Date:
Date Created: 20190613 Date Completed: 20191107 Latest Revision: 20231012
- Publication Date:
20231215
- Accession Number:
PMC6558854
- Accession Number:
10.1186/s12864-019-5790-2
- Accession Number:
31185912
No Comments.