Regulation of Low and High Nucleic Acid Fluorescent Heterotrophic Prokaryote Subpopulations and Links to Viral-Induced Mortality Within Natural Prokaryote-Virus Communities.

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  • Additional Information
    • Source:
      Publisher: Springer-Verlag Country of Publication: United States NLM ID: 7500663 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-184X (Electronic) Linking ISSN: 00953628 NLM ISO Abbreviation: Microb Ecol Subsets: MEDLINE
    • Publication Information:
      Original Publication: New York, Springer-Verlag.
    • Subject Terms:
      Virus Physiological Phenomena*; Archaea/*genetics ; Bacteria/*genetics ; Prokaryotic Cells/*metabolism ; Prokaryotic Cells/*virology; Archaea/chemistry ; Archaea/metabolism ; Archaea/virology ; Bacteria/chemistry ; Bacteria/metabolism ; Bacteria/virology ; Carbon/metabolism ; Flow Cytometry ; Fluorescence ; Heterotrophic Processes ; Host-Pathogen Interactions ; Nucleic Acids/chemistry ; Nucleic Acids/genetics ; Prokaryotic Cells/chemistry ; Viruses/genetics ; Viruses/growth & development
    • Abstract:
      Flow cytometric analysis of marine prokaryotes routinely reveals two distinct clusters of heterotrophic cells referred to as high nucleic acid fluorescent (HNA) and low nucleic acid fluorescent (LNA) populations. Evidence suggests that these may represent physiologically and ecologically distinct prokaryote populations. According to the "kill the winner" hypothesis, viral lysis reduces the efficiency of the microbial loop by decreasing the biomass and activity of the most abundant and active members of a population (i.e., competition specialist). Thus, viral-induced mortality may vary according to the physiology of HNA and LNA cells, with implications for the marine carbon cycle. Here, the abundance and production of heterotrophic prokaryotic populations were assessed in the North Atlantic during two phases of the annual plankton cycle and related to bottom-up (i.e., organic carbon variability) and top-down processes (i.e., viral abundance and lytic production). Our results demonstrate that the relative abundance of HNA and LNA heterotrophic cells and heterotrophic prokaryote production vary according to organic carbon variability in the water column, which can be strongly influenced by the physical eddy field (i.e., type of eddy: cyclonic, anticyclonic, or no eddy). In addition, the abundance and lytic production of virus subpopulations were correlated with  the cellular production and abundance of heterotrophic HNA and LNA prokaryote communities. Our data suggest group- and activity-specific linkages between hosts and viruses (i.e., HNA-V1 and LNA-V2). Specifically, V1 had a greater contribution to total viral production (i.e., 2.6-fold higher than V2 viruses), similar to their putative host. Finally, we explore potential implications of group- and activity-specific linkages between host and virus groups on the flux of carbon through the microbial food web.
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    • Grant Information:
      NNX15AF30G United States NASA NASA; 1537943 National Science Foundation
    • Contributed Indexing:
      Keywords: Flow cytometry; High nucleic acid bacteria; Low nucleic acid bacteria; Marine viruses; Viral lysis
    • Accession Number:
      0 (Nucleic Acids)
      7440-44-0 (Carbon)
    • Publication Date:
      Date Created: 20190605 Date Completed: 20200527 Latest Revision: 20200527
    • Publication Date:
      20221213
    • Accession Number:
      10.1007/s00248-019-01393-9
    • Accession Number:
      31161232