Differential Anti-Adipogenic Effects of Eicosapentaenoic and Docosahexaenoic Acids in Obesity.

Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 101231818 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1613-4133 (Electronic) Linking ISSN: 16134125 NLM ISO Abbreviation: Mol Nutr Food Res Subsets: MEDLINE

Original Publication: Weinheim, Germany : Wiley-VCH, c2004-

Adipogenesis/*physiology ; Docosahexaenoic Acids/*blood ; Eicosapentaenoic Acid/*blood ; Obesity/*blood; 3T3-L1 Cells ; Adipogenesis/drug effects ; Adipose Tissue, White/drug effects ; Adipose Tissue, White/physiology ; Animals ; Anti-Obesity Agents/pharmacology ; Body Fat Distribution ; Diet, High-Fat/adverse effects ; Dietary Supplements ; Docosahexaenoic Acids/pharmacology ; Eicosapentaenoic Acid/pharmacology ; Female ; Humans ; Male ; Mice ; Mice, Inbred C57BL ; Middle Aged ; Nutrition Surveys ; Obesity/etiology ; United States

Scope: To assess the associations of plasma eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) with body fat in a population-based sample and explore the mechanism of action based on browning of white adipose tissue (WAT) in high-fat-diet-induced obese (DIO) mice and 3T3-L1 adipocytes.
Methods and Results: Plasma EPA and DHA of 1719 adults in the National Health and Nutrition Examination Survey (2003-2004) are determined by gas chromatography mass spectrometry, while total body fat is measured by dual-energy X-ray absorptiometry. DIO mice are fed a high-fat diet supplemented with EPA or DHA (1% wt/wt) for 15 weeks and 3T3-L1 preadipocytes are treated with EPA or DHA during differentiation. Plasma DHA but not EPA is associated with lower body fat mass (p trend < 0.0001), which persists in overweight/obese subjects (p trend = 0.02). DHA supplementation reduces inguinal WAT and exhibits a more pronounced thermogenic effect than EPA in DIO mice. In vitro, the browning process is induced after 2-day and 6-day treatment with DHA and EPA, respectively.
Conclusion: Plasma DHA but not EPA is inversely associated with body fat mass. The more potent anti-adipogenic effect of DHA than EPA may involve a better capability of inducing browning of WAT for DHA.
(© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

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Keywords: adiposity; browning of white adipose; docosahexaenoic acid; eicosapentaenoic acid

0 (Anti-Obesity Agents)
25167-62-8 (Docosahexaenoic Acids)
AAN7QOV9EA (Eicosapentaenoic Acid)

Date Created: 20190530 Date Completed: 20201113 Latest Revision: 20201113

20221213

10.1002/mnfr.201801135

31140724