Prevalence of Hepatitis B Virus, Genotypes, and Mutants in HBsAg-Positive Patients in Meerut, India

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  • Author(s): Khan S;Khan S; Madan M; Madan M; Virmani; Virmani
  • Source:
    Iranian biomedical journal [Iran Biomed J] 2019 Sep; Vol. 23 (5), pp. 354-61. Date of Electronic Publication: 2019 May 19.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Pasteur Institute of Iran Country of Publication: Iran NLM ID: 9814853 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2008-823X (Electronic) Linking ISSN: 1028852X NLM ISO Abbreviation: Iran Biomed J Subsets: MEDLINE
    • Publication Information:
      Original Publication: Tehran : Pasteur Institute of Iran
    • Subject Terms:
      Hepatitis B Surface Antigens/*genetics ; Hepatitis B virus/*genetics ; Mutation/*genetics; Adult ; Aged ; Base Sequence ; DNA, Viral/genetics ; Female ; Genotype ; Humans ; India ; Male ; Middle Aged ; Prevalence ; Viral Load ; Young Adult
    • Abstract:
      Background: Genetic changeability of hepatitis B virus (HBV) signifies a challenge for the sensitivity of immunologic and molecular diagnostics. Therefore, knowing the spread of HBV genotypes (GENs) and mutation has considerable impacts on treatment strategies, vaccination program, diagnosis, and prevention. The present study aimed to detect HBV GENs and mutants in HBsAg-positive patients.
      Methods: The study conducted on 4927 patients in Meerut, India, between March 2013 and April 2017. The blood specimens were analyzed for HBsAg using an ELISA kit, then the blood samples from HBsAg-positive patients were subjected to HBeAg assay and DNA isolation. Amplification of the HBV DNA of pre-S gene and pre-core or basal core promoter region were performed by RT-PCR and sequenced to analyze both GEN and mutation.
      Results: According to the results, 245 cases were positive for HBsAg, and 55 were HBeAg-positive. With regard to HBV DNA levels, 16 samples were found positive in PCR assay with 7 (43.8%) less than 2000 IU/mL, 4 (25%) between >2000 and 20,000 IU/mL, and 5 (3.25%) >20,000 IU/mL. No mutations were detected in GENs B and A. The prevalence of HBV GENs B and A were 68.8% (n = 11) and 31.25% (n = 6), respectively.
      Conclusion: GEN-B was more prevalent in comparison to GEN-A. The genetic diversity of HBV and distribution of its GENs and mutation improve the current knowledge of epidemiological, clinical and virological patterns of hepatitis B in this region, which help physicians to prescribe proper antiviral/interferon therapy according to current genotyping pattern.
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    • Contributed Indexing:
      Keywords: Genotype; Hepatitis B surface antigens; Hepatitis B virus; India; Mutation
    • Accession Number:
      0 (DNA, Viral)
      0 (Hepatitis B Surface Antigens)
    • Publication Date:
      Date Created: 20190520 Date Completed: 20191212 Latest Revision: 20200225
    • Publication Date:
      20221213
    • Accession Number:
      PMC6661126
    • Accession Number:
      31103024