Proteome of the central apparatus of a ciliary axoneme.

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  • Additional Information
    • Source:
      Publisher: Rockefeller University Press Country of Publication: United States NLM ID: 0375356 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1540-8140 (Electronic) Linking ISSN: 00219525 NLM ISO Abbreviation: J Cell Biol Subsets: MEDLINE
    • Publication Information:
      Original Publication: New York : Rockefeller University Press
    • Subject Terms:
      Algal Proteins/*metabolism ; Axoneme/*metabolism ; Chlamydomonas/*metabolism ; Cilia/*metabolism ; Flagella/*metabolism ; Microtubule Proteins/*metabolism ; Proteome/*analysis; Algal Proteins/genetics ; Cell Movement ; Chlamydomonas/genetics ; Chlamydomonas/growth & development ; Mass Spectrometry ; Microtubule Proteins/genetics ; Mutation ; Proteome/isolation & purification
    • Abstract:
      Nearly all motile cilia have a "9+2" axoneme containing a central apparatus (CA), consisting of two central microtubules with projections, that is essential for motility. To date, only 22 proteins are known to be CA components. To identify new candidate CA proteins, we used mass spectrometry to compare axonemes of wild-type Chlamydomonas and a CA-less mutant. We identified 44 novel candidate CA proteins, of which 13 are conserved in humans. Five of the latter were studied more closely, and all five localized to the CA; therefore, most of the other candidates are likely to also be CA components. Our results reveal that the CA is far more compositionally complex than previously recognized and provide a greatly expanded knowledge base for studies to understand the architecture of the CA and how it functions. The discovery of the new conserved CA proteins will facilitate genetic screening to identify patients with a form of primary ciliary dyskinesia that has been difficult to diagnose.
      (© 2019 Zhao et al.)
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    • Grant Information:
      R35 GM122574 United States GM NIGMS NIH HHS; R37 GM030626 United States GM NIGMS NIH HHS
    • Molecular Sequence:
      GENBANK AAK38270; AAZ31187; AAZ31185; AAZ31184; ADD85930; ADD85929; AAD38856; ACR55627
      RefSeq XP_001696622; XP_001702926; XP_001697065; XP_001703508; XP_001702971; XP_001701326; XP_001702776; XP_001696950; XP_001690036; XP_001689997; XP_001701617; XP_001694680; XP_001693026; XP_001694877; XP_001691575; XP_001696011; XP_001702074; XP_001692552; XP_001696096; XP_001696434; XP_001694909; XP_001691318; XP_001693652; XP_001692825; XP_001691070; XP_001691315; XP_001703476; XP_001693959; XP_001694913; XP_001692304; XP_001696012; XP_001692822; XP_001702799; XP_001697045; XP_001702365; XP_001689770; XP_001695620; XP_001689839; XP_001699180; XP_001699181; XP_001697713; XP_001690861; XP_001700259; XP_001692669; XP_001701140; XP_001699354; XP_001689865; XP_001696779; XP_001702388; XP_001696110; XP_001694676; XP_001698357; XP_001691634; XP_001702692; XP_001697145; XP_001695420; XP_001697337; XP_001703266; XP_001697178
      SWISSPROT P93107
    • Accession Number:
      0 (Algal Proteins)
      0 (Microtubule Proteins)
      0 (Proteome)
    • Publication Date:
      Date Created: 20190517 Date Completed: 20200409 Latest Revision: 20230701
    • Publication Date:
      20230701
    • Accession Number:
      PMC6548120
    • Accession Number:
      10.1083/jcb.201902017
    • Accession Number:
      31092556