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Comparative Loss-of-Function Screens Reveal ABCE1 as an Essential Cellular Host Factor for Efficient Translation of Paramyxoviridae and Pneumoviridae .
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- Author(s): Anderson DE;Anderson DE; Pfeffermann K; Pfeffermann K; Kim SY; Kim SY; Kim SY; Sawatsky B; Sawatsky B; Pearson J; Pearson J; Pearson J; Kovtun M; Kovtun M; Corcoran DL; Corcoran DL; Krebs Y; Krebs Y; Sigmundsson K; Sigmundsson K; Jamison SF; Jamison SF; Yeo ZZJ; Yeo ZZJ; Rennick LJ; Rennick LJ; Wang LF; Wang LF; Talbot PJ; Talbot PJ; Duprex WP; Duprex WP; Garcia-Blanco MA; Garcia-Blanco MA; Garcia-Blanco MA; Garcia-Blanco MA; Garcia-Blanco MA; von Messling V; von Messling V; von Messling V; von Messling V
- Source:
MBio [mBio] 2019 May 14; Vol. 10 (3). Date of Electronic Publication: 2019 May 14.- Publication Type:
Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't- Language:
English - Source:
- Additional Information
- Source: Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 101519231 Publication Model: Electronic Cited Medium: Internet ISSN: 2150-7511 (Electronic) NLM ISO Abbreviation: mBio Subsets: MEDLINE
- Publication Information: Original Publication: Washington, D.C. : American Society for Microbiology
- Subject Terms:
- Abstract: Paramyxoviruses and pneumoviruses have similar life cycles and share the respiratory tract as a point of entry. In comparative genome-scale siRNA screens with wild-type-derived measles, mumps, and respiratory syncytial viruses in A549 cells, a human lung adenocarcinoma cell line, we identified vesicular transport, RNA processing pathways, and translation as the top pathways required by all three viruses. As the top hit in the translation pathway, ABCE1, a member of the ATP-binding cassette transporters, was chosen for further study. We found that ABCE1 supports replication of all three viruses, confirming its importance for viruses of both families. More detailed characterization revealed that ABCE1 is specifically required for efficient viral but not general cellular protein synthesis, indicating that paramyxoviral and pneumoviral mRNAs exploit specific translation mechanisms. In addition to providing a novel overview of cellular proteins and pathways that impact these important pathogens, this study highlights the role of ABCE1 as a host factor required for efficient paramyxovirus and pneumovirus translation. IMPORTANCE The Paramyxoviridae and Pneumoviridae families include important human and animal pathogens. To identify common host factors, we performed genome-scale siRNA screens with wild-type-derived measles, mumps, and respiratory syncytial viruses in the same cell line. A comparative bioinformatics analysis yielded different members of the coatomer complex I, translation factors ABCE1 and eIF3A, and several RNA binding proteins as cellular proteins with proviral activity for all three viruses. A more detailed characterization of ABCE1 revealed its essential role for viral protein synthesis. Taken together, these data sets provide new insight into the interactions between paramyxoviruses and pneumoviruses and host cell proteins and constitute a starting point for the development of broadly effective antivirals.
(Copyright © 2019 Anderson et al.) - References: Nucleic Acids Res. 2018 Sep 6;46(15):7820-7830. (PMID: 30010948)
Cell Microbiol. 2015 Jan;17(1):131-45. (PMID: 25154738)
J Virol. 1984 Jul;51(1):71-6. (PMID: 6726896)
Cell Host Microbe. 2017 Oct 11;22(4):460-470.e5. (PMID: 29024641)
Nature. 2012 Feb 22;482(7386):501-6. (PMID: 22358840)
J Cell Biol. 2012 Aug 6;198(3):439-56. (PMID: 22851315)
Clin Chem. 2003 Feb;49(2):230-8. (PMID: 12560344)
PLoS Pathog. 2016 Mar 24;12(3):e1005478. (PMID: 27010548)
Nat Methods. 2009 Aug;6(8):569-75. (PMID: 19644458)
Vaccine. 2016 Jun 24;34(30):3525-34. (PMID: 27154393)
Virology. 2016 Nov;498:94-98. (PMID: 27567257)
Biochem Biophys Res Commun. 2004 Feb 27;315(1):166-73. (PMID: 15013441)
Mol Cell Proteomics. 2015 Mar;14(3):532-43. (PMID: 25556234)
J Virol. 2017 Jan 31;91(4):. (PMID: 27974556)
Proc Natl Acad Sci U S A. 2007 Jun 5;104(23):9585-90. (PMID: 17535916)
Eur J Biochem. 1998 Jun 1;254(2):248-55. (PMID: 9660177)
Virology. 2002 Jan 5;292(1):70-7. (PMID: 11878909)
Virology. 1997 May 26;232(1):44-52. (PMID: 9185587)
Nat Rev Genet. 2015 May;16(5):299-311. (PMID: 25854182)
Virus Res. 1986 Jul;5(1):77-95. (PMID: 3755855)
PLoS One. 2015 Feb 06;10(2):e0116702. (PMID: 25659154)
Curr Opin Virol. 2012 Dec;2(6):784-92. (PMID: 23122855)
Cell. 2013 Jun 20;153(7):1589-601. (PMID: 23791185)
J Virol. 2014 Aug;88(15):8355-60. (PMID: 24829348)
J Virol. 2000 May;74(9):4236-43. (PMID: 10756037)
J Biol Chem. 2004 Oct 1;279(40):42157-68. (PMID: 15277527)
J Virol. 2015 Mar;89(5):2849-56. (PMID: 25540371)
Emerg Infect Dis. 2014 Mar;20(3):372-9. (PMID: 24572697)
Pediatrics. 1992 Jul;90(1 Pt 2):149-53. (PMID: 1603640)
J Biol Chem. 2006 Feb 17;281(7):3773-84. (PMID: 16275648)
Commun Integr Biol. 2017 May 8;10(3):e1319025. (PMID: 28702128)
Cell. 2015 Aug 13;162(4):872-84. (PMID: 26276635)
Proc Natl Acad Sci U S A. 2013 Jan 2;110(1):324-9. (PMID: 23169626)
Front Microbiol. 2011 Aug 23;2:173. (PMID: 22007186)
Arch Virol. 2014 Jun;159(6):1267-77. (PMID: 24343264)
PLoS One. 2012;7(12):e51942. (PMID: 23251657)
Nat Rev Microbiol. 2008 May;6(5):363-74. (PMID: 18414501)
Nat Struct Mol Biol. 2011 Mar;18(3):372-8. (PMID: 21336277)
Curr Opin Microbiol. 2009 Aug;12(4):446-52. (PMID: 19576842)
J Biol Chem. 2010 Jul 30;285(31):24023-31. (PMID: 20519500)
Nat Rev Mol Cell Biol. 2014 Sep;15(9):591-600. (PMID: 25145850)
Virology. 2008 Feb 20;371(2):227-30. (PMID: 18177685)
J Virol. 2006 Mar;80(6):2987-99. (PMID: 16501108)
PLoS Pathog. 2011 Jan 27;7(1):e1001263. (PMID: 21304593)
Arch Virol. 2016 Aug;161(8):2351-60. (PMID: 27216929)
J Biol Chem. 2006 Mar 17;281(11):7452-7. (PMID: 16421098)
J Virol. 2013 Feb;87(3):1333-47. (PMID: 23152511)
IUBMB Life. 2012 Oct;64(10):795-800. (PMID: 23008114)
J Virol. 2009 Jan;83(2):961-8. (PMID: 19004947)
Nature. 2010 Feb 11;463(7282):813-7. (PMID: 20027183)
J Virol. 2012 Jan;86(2):655-66. (PMID: 22072764)
J Virol. 2014 Jan;88(1):456-68. (PMID: 24155404)
Cold Spring Harb Perspect Biol. 2013 Feb 01;5(2):. (PMID: 23378591)
J Virol. 2002 Aug;76(16):8244-51. (PMID: 12134030)
Mol Cell. 2010 Jan 29;37(2):196-210. (PMID: 20122402)
Virology. 2015 May;479-480:532-44. (PMID: 25824479)
PLoS Pathog. 2006 Oct;2(10):e102. (PMID: 17040126)
J Virol. 2008 Jan;82(2):828-39. (PMID: 17977969)
J Biomol Screen. 2014 Jul;19(6):936-46. (PMID: 24464431)
J Virol. 2015 Oct;89(19):10115-9. (PMID: 26178980)
J Am Vet Med Assoc. 1966 Sep 1;149(5):662-5. (PMID: 6008148)
Vaccine. 2013 Apr 18;31 Suppl 2:B209-15. (PMID: 23598484)
Mol Cell Proteomics. 2010 Sep;9(9):1829-48. (PMID: 20530633)
Nature. 2002 Jan 3;415(6867):88-92. (PMID: 11780123)
J Biol Chem. 2001 Dec 21;276(51):48473-82. (PMID: 11585831)
J Virol. 1987 Feb;61(2):584-9. (PMID: 3027385)
Nat Commun. 2016 Nov 08;7:13248. (PMID: 27824037)
Anal Chem. 2013 Jan 15;85(2):898-906. (PMID: 23205519)
Biochimie. 2007 Jun-Jul;89(6-7):789-98. (PMID: 17400356)
PLoS Pathog. 2016 Dec 7;12(12):e1006062. (PMID: 27926942)
Traffic. 2007 Mar;8(3):195-211. (PMID: 17233757)
Proc Natl Acad Sci U S A. 2011 Nov 22;108(47):19036-41. (PMID: 22065774)
Antiviral Res. 2018 Feb;150:123-129. (PMID: 29258862)
MMWR Morb Mortal Wkly Rep. 2010 Feb 12;59(5):125-9. (PMID: 20150887)
PLoS Negl Trop Dis. 2012;6(9):e1820. (PMID: 23029581)
J Microbiol Immunol Infect. 2005 Oct;38(5):327-31. (PMID: 16211140)
Cell Host Microbe. 2014 Jul 9;16(1):19-30. (PMID: 25011105)
J Virol. 1999 Jan;73(1):290-6. (PMID: 9847332)
J Virol. 2003 Dec;77(23):12579-91. (PMID: 14610181)
Cell. 1993 Mar 12;72(5):753-65. (PMID: 7680958)
J Biol Chem. 1995 Jun 2;270(22):13308-17. (PMID: 7539425)
J Biomol Screen. 2010 Aug;15(7):735-47. (PMID: 20625183)
Rev Sci Tech. 2012 Dec;31(3):729-46. (PMID: 23520729) - Grant Information: R01 AI089526 United States AI NIAID NIH HHS; R01 AI099100 United States AI NIAID NIH HHS; R01 AI101431 United States AI NIAID NIH HHS; MOP-66989 Canada CIHR
- Contributed Indexing: Keywords: ABCE1; Paramyxoviridae; Pneumoviridae; RNAi screen; host factor; respiratory syncytial virus
- Accession Number: 0 (ABCE1 protein, human)
0 (ATP-Binding Cassette Transporters)
0 (RNA, Messenger)
0 (RNA, Small Interfering)
0 (RNA-Binding Proteins) - Publication Date: Date Created: 20190516 Date Completed: 20190705 Latest Revision: 20200309
- Publication Date: 20221213
- Accession Number: PMC6520455
- Accession Number: 10.1128/mBio.00826-19
- Accession Number: 31088929
- Source:
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