Opisthorchis felineus infection provokes time-dependent accumulation of oxidative hepatobiliary lesions in the injured hamster liver.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read Online Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
    • Publication Information:
      Original Publication: San Francisco, CA : Public Library of Science
    • Subject Terms:
      Oxidative Stress*; Digestive System Diseases/*complications ; Digestive System Diseases/*metabolism ; Liver/*metabolism ; Liver/*pathology ; Opisthorchiasis/*complications ; Opisthorchis/*physiology; Animals ; Biomarkers/metabolism ; Cricetinae ; DNA Damage ; Digestive System Diseases/genetics ; Digestive System Diseases/pathology ; Disease Models, Animal ; Lipid Peroxidation ; Time Factors
    • Abstract:
      Opisthorchiasis caused by food-borne trematode Opisthorchis felineus is a substantial public health problem, with 17 million persons infected worldwide. This chronic disease is associated with hepatobiliary inflammation, cholangiocyte dysplasia, cholangiofibrosis, intraepithelial neoplasia, and even cholangiocarcinoma among chronically infected individuals. To provide first insights into the mechanism by which O. felineus infection causes precancerous liver lesions, we investigated the level of oxidative stress (lipid peroxidation byproducts and 8-hydroxy-2'-deoxyguanosine) as well as the time course profiles of chronic inflammation and fibrogenesis markers in the dynamics of opisthorchiasis from 1 month to 1.5 years postinfection in an experimental model based on golden hamsters Mesocricetus auratus. For the first time, we showed that O. felineus infection provokes time-dependent accumulation of oxidative hepatobiliary lesions in the injured liver of hamsters. In particular, over the course of infection, lipid peroxidation byproducts 4-hydroxynonenal and malondialdehyde were upregulated; these changes in general correlate with the dynamics of hepatic histopathological changes. We detected macrophages with various immunophenotypes and elevated levels of CD68, COX2, and CD163 in the O. felineus-infected animals. Meanwhile, there was direct time-dependent elevation of TNF-α (R = 0.79; p < 0.001) and CD163 protein levels (R = 0.58; p = 0.022). We also provide quantitative data about epithelial hyperplasia marker CK7 and a marker of myofibroblast activation (α smooth muscle actin). Our present data provide first insights into the histopathological mechanism by which O. felineus infection causes liver injuries. These findings support the inclusion of O. felineus in Group 1 of biological carcinogens.
      Competing Interests: The authors have declared that no competing interests exist.
    • References:
      Int J Parasitol. 2016 Mar;46(3):195-204. (PMID: 26718397)
      PLoS Negl Trop Dis. 2015 Dec 01;9(12):e0004258. (PMID: 26625139)
      Front Immunol. 2015 Nov 25;6:602. (PMID: 26635814)
      Antioxid Redox Signal. 2004 Feb;6(1):19-24. (PMID: 14713333)
      Clin Lipidol. 2012 Oct 1;7(5):537-548. (PMID: 23630545)
      Adv Parasitol. 2018;102:97-113. (PMID: 30442312)
      Trans R Soc Trop Med Hyg. 2016 Jan;110(1):28-36. (PMID: 26740360)
      Parasite Immunol. 2014 Jun;36(6):233-52. (PMID: 24666543)
      Oxid Med Cell Longev. 2014;2014:360438. (PMID: 24999379)
      Lipids. 2000 Sep;35(9):961-5. (PMID: 11026616)
      IARC Monogr Eval Carcinog Risks Hum. 2012;100(Pt B):1-441. (PMID: 23189750)
      Asian Pac J Cancer Prev. 2015;16(7):3043-50. (PMID: 25854403)
      Antioxid Redox Signal. 2006 May-Jun;8(5-6):1047-58. (PMID: 16771694)
      PLoS Med. 2007 Jul;4(7):e201. (PMID: 17622191)
      Parasitol Int. 2017 Feb;66(1):889-892. (PMID: 27769807)
      Bull Exp Biol Med. 2017 Apr;162(6):773-776. (PMID: 28429227)
      Carcinogenesis. 2017 Sep 1;38(9):929-937. (PMID: 28910999)
      Parasitol Int. 2012 Mar;61(1):25-31. (PMID: 21840415)
      Acta Trop. 2013 Apr;126(1):54-62. (PMID: 23337391)
      Infect Immun. 2017 Mar 23;85(4):. (PMID: 28138021)
      PLoS Pathog. 2015 Oct 20;11(10):e1005209. (PMID: 26485648)
      Mutat Res. 2012 Mar 1;731(1-2):48-57. (PMID: 22044627)
      Immunity. 2017 Dec 19;47(6):1024-1036. (PMID: 29262347)
      J Environ Sci Health C Environ Carcinog Ecotoxicol Rev. 2009 Apr;27(2):120-39. (PMID: 19412858)
      Parasitol Int. 2017 Aug;66(4):458-463. (PMID: 26453019)
      Am J Trop Med Hyg. 2013 Feb;88(2):364-6. (PMID: 23249682)
    • Accession Number:
      0 (Biomarkers)
    • Publication Date:
      Date Created: 20190516 Date Completed: 20200117 Latest Revision: 20200309
    • Publication Date:
      20231215
    • Accession Number:
      PMC6516637
    • Accession Number:
      10.1371/journal.pone.0216757
    • Accession Number:
      31086416