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Formation of an Organic-Inorganic Biopolymer: Polyhydroxybutyrate-Polyphosphate.
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- Additional Information
- Source:
Publisher: American Chemical Society Country of Publication: United States NLM ID: 100892849 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1526-4602 (Electronic) Linking ISSN: 15257797 NLM ISO Abbreviation: Biomacromolecules Subsets: MEDLINE
- Publication Information:
Original Publication: Washington, DC : American Chemical Society, c2000-
- Subject Terms:
- Abstract:
A considerable variety of different biopolymers is formed by the entirety of organisms present on earth. Most of these compounds are organic polymers such as polysaccharides, polyamino acids, polynucleotides, polyisoprenes or polyhydroxyalkanoates (PHAs), but some biopolymers can consist of solely inorganic monomers such as phosphate in polyphosphates (polyPs). In this contribution, we describe the formation of an organic-inorganic block copolymer consisting of poly(3-hydroxybutyrate) (PHB) and polyP. This was achieved by the expression of a fusion of the polyP kinase gene ( ppk2c ) with the PHB synthase gene ( phaC ) of Ralstonia eutropha in a polyP-free and PHB-free mutant background of R. eutropha . The fusion protein catalyzed both the formation of polyP by its polyP kinase domain and the formation of PHB by its PHB synthase domain. It was also possible to synthesize the polyP-PHB polymer in vitro with purified Ppk2c-PhaC, if the monomers, adenosine triphosphate (ATP) and 3-hydroxybutyryl-CoA (3HB-CoA), were provided. Most likely, the formed block copolymer (polyP-protein-PHB) turns into a blend of polyP and PHB after release from the enzyme.
- Accession Number:
0 (Bacterial Proteins)
0 (Biopolymers)
0 (Polyesters)
0 (Polyhydroxyalkanoates)
0 (Polyphosphates)
EC 2.3.- (Acyltransferases)
EC 2.3.1.- (poly-beta-hydroxybutyrate polymerase)
- Publication Date:
Date Created: 20190508 Date Completed: 20200908 Latest Revision: 20200908
- Publication Date:
20221213
- Accession Number:
10.1021/acs.biomac.9b00208
- Accession Number:
31062966
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