A loop-mediated isothermal amplification coupling with a lateral flow dipstick for rapid and specific detection of fowl adenovirus serotype-4.

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  • Additional Information
    • Source:
      Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0984 (Electronic) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE
    • Publication Information:
      Original Publication: [Amsterdam] : Elsevier/North-Holland Biomedical Press, 1980-
    • Subject Terms:
      Adenoviridae/*isolation & purification ; Chickens/*virology ; Chromatography/*veterinary ; Nucleic Acid Amplification Techniques/*veterinary; Adenoviridae/genetics ; Animals ; DNA Primers/genetics ; Real-Time Polymerase Chain Reaction/veterinary ; Sensitivity and Specificity ; Serogroup
    • Abstract:
      Fowl adenovirus serotype-4 (FAdV-4) has been recognized as a predominant threat to the broilers aged from three to five weeks. Hydropericardium syndrome (HPS) is one of its major clinical diseases by FAdV-4 resulting in heavy economic losses. In this study, a loop-mediated isothermal amplification coupling with a lateral flow dipstick (LAMP-LFD) was developed for rapid and specific detection of fowl adenovirus serotype-4. The optimized LAMP-LFD can be completed in 60 min at 65 °C. The minimum detection limits of PCR, real-time PCR, nested PCR and LAMP-LFD are 1 × 10 4 copies/μl, 1 × 10 2 copies/μl, 10 copies/μl and 10 copies/μl respectively. Moreover, the specificity of the LAMP-LFD assay is satisfactory and does not produce cross reactions with other species. In field samples, 150 samples were assayed by PCR and LAMP-LFD. They agreed on the diagnosis "positive" in 13% of clinical samples, and they agreed on the diagnosis "negative" in 85% of clinical samples. Their probability of agreement is p 0  = 147/150 = 13% + 85% = 98%. LAMP-LFD can potentially be modified and applied as a diagnostic tool for FAdV-4 infection especially in resource-limited areas, such as small breeding farms and basic veterinary labs to offer an affordable diagnostic.
      (Copyright © 2019 Elsevier B.V. All rights reserved.)
    • Contributed Indexing:
      Keywords: Diagnostic; Fowl adenovirus; LAMP; Lateral flow dipstick
    • Accession Number:
      0 (DNA Primers)
    • Publication Date:
      Date Created: 20190505 Date Completed: 20200625 Latest Revision: 20200625
    • Publication Date:
      20221213
    • Accession Number:
      10.1016/j.jviromet.2019.04.026
    • Accession Number:
      31054280