Protective Effects of Moderate Ca Supplementation against Cd-Induced Bone Damage under Different Population-Relevant Doses in Young Female Rats.

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  • Additional Information
    • Source:
      Publisher: MDPI Publishing Country of Publication: Switzerland NLM ID: 101521595 Publication Model: Electronic Cited Medium: Internet ISSN: 2072-6643 (Electronic) Linking ISSN: 20726643 NLM ISO Abbreviation: Nutrients Subsets: MEDLINE
    • Publication Information:
      Original Publication: Basel, Switzerland : MDPI Publishing
    • Subject Terms:
    • Abstract:
      Estimation of the skeleton-protective effects of Ca in Cd-induced bone damage is helpful in the assessment of Cd health risk. The aim of this study was to identify whether Ca supplementation during exposure to different population-relevant doses of Cd can prevent Cd-induced bone damage under the tolerable upper intake level of Ca supplementation. Young female Sprague-Dawley rats were given different population-relevant doses of Cd (1, 5, and 50 mg Cd/kg diet) and Ca supplementation (0.4% Ca supplementation) intervention. Ca supplementation significantly decreased Cd-induced bone microstructure damage, increased bone biomechanics ( p < 0.05), serum bone formation marker level ( p < 0.05) and expression of osteogenic gene markers exposure to the 5 and 50 mg Cd/kg diets. However, it had no impact on these indicators under the 1 mg Cd/kg diets, with the exception of expression of osteogenic marker genes. Ca supplementation significantly decreased serum Klotho level ( p < 0.05), and fibroblast growth factor 23/Klotho-associated gene expression in the kidney and bone showed significant changes. In conclusion, Ca supplementation has a positive effect on bone formation and bone quality against the damaging impact of Cd, especially with exposure to the 5 mg and 50 mg Cd/kg diet, which may be related to its impact on the fibroblast growth factor 23/Klotho axis.
      Competing Interests: The authors declare no conflict of interest.
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    • Grant Information:
      81673171 the National Natural Science Foundation of China; BE2015646 Social Development Research Program of Jiangsu Province Science and Technology department
    • Contributed Indexing:
      Keywords: bone; cadmium; calcium; fibroblast growth factor 23; klotho
    • Accession Number:
      0 (Biomarkers)
      00BH33GNGH (Cadmium)
      62031-54-3 (Fibroblast Growth Factors)
      7Q7P4S7RRE (Fibroblast Growth Factor-23)
      EC 3.2.1.31 (Glucuronidase)
      EC 3.2.1.31 (Klotho Proteins)
      SY7Q814VUP (Calcium)
    • Publication Date:
      Date Created: 20190418 Date Completed: 20190905 Latest Revision: 20240716
    • Publication Date:
      20240716
    • Accession Number:
      PMC6521033
    • Accession Number:
      10.3390/nu11040849
    • Accession Number:
      30991710